AI SearchEnzymatic preparation of chitin oligosaccharides
Ilankovan, Paraman
Enzymatic preparation of chitin oligosaccharides - Bangkok : Asian Institute of Technology, 2002 - 74 leaves - Thesis ; no. BP-02-09 . - Asian Institute of Technology. Thesis ; no. BP-02-09 .
A thesis submitted in partial fulfillment of the requirements for the degree of Mater of Science, School of Environment, Resources and Development
Thesis (M.Sc.) - Asian Institute of Technology, 2002
The aim of this study is to produce N-acetyl glucosamine and water-soluble Nacetyl chito oligosaccharides using commercially available enzymes. There are altogether 20 enzymes examined. Pepsin and cellulase showed comparatively higher chitinolytic activity among the tested non-specific enzymes. Pepsin primarily produced dimer and small amount of monomer and trimer units. Cellulase mainly produced monomer units. High c1ystallinity of chitin is the major factor which limits the enzymatic hydrolysis to proceed. Modifications on chitin were important for partial destruction of crystallinity. The products yield from enzymatic hydrolysis was much better in decrystallized chitins compared to non-decrystallized natural chitins. Especially the suspension forms of the decrystallized chitins were more susceptible to enzymatic hydrolysis than solid form of chitins. The modified chitins were found to be more susceptible to enzymatic degradation. Alkaline treatment to produced amorphous chitin, phosphoric acid treatment, production of super fine chitin were the methods significantly reduced the crystallinity of chitin. Hydrochloric acid treatment did not show significant reduction in crystalline nature of chitin. Enzymatic reactions on two type chitins, a. chitin from shrimp, crab and
Chitin
Chitosan
Enzymes
Enzymatic preparation of chitin oligosaccharides - Bangkok : Asian Institute of Technology, 2002 - 74 leaves - Thesis ; no. BP-02-09 . - Asian Institute of Technology. Thesis ; no. BP-02-09 .
A thesis submitted in partial fulfillment of the requirements for the degree of Mater of Science, School of Environment, Resources and Development
Thesis (M.Sc.) - Asian Institute of Technology, 2002
The aim of this study is to produce N-acetyl glucosamine and water-soluble Nacetyl chito oligosaccharides using commercially available enzymes. There are altogether 20 enzymes examined. Pepsin and cellulase showed comparatively higher chitinolytic activity among the tested non-specific enzymes. Pepsin primarily produced dimer and small amount of monomer and trimer units. Cellulase mainly produced monomer units. High c1ystallinity of chitin is the major factor which limits the enzymatic hydrolysis to proceed. Modifications on chitin were important for partial destruction of crystallinity. The products yield from enzymatic hydrolysis was much better in decrystallized chitins compared to non-decrystallized natural chitins. Especially the suspension forms of the decrystallized chitins were more susceptible to enzymatic hydrolysis than solid form of chitins. The modified chitins were found to be more susceptible to enzymatic degradation. Alkaline treatment to produced amorphous chitin, phosphoric acid treatment, production of super fine chitin were the methods significantly reduced the crystallinity of chitin. Hydrochloric acid treatment did not show significant reduction in crystalline nature of chitin. Enzymatic reactions on two type chitins, a. chitin from shrimp, crab and
Chitin
Chitosan
Enzymes