AI SearchEstrogenic activities of sesame lignans and their metabolites : interactive effect with cadmium in human breast cancer cell lines
Prisna Pianjing
Estrogenic activities of sesame lignans and their metabolites : interactive effect with cadmium in human breast cancer cell lines - Pathum Thani, Thailand : Asian Institute of Technology, 2011 - 91 leaves : ill. (some col.) - Dissertation ; no. EV-11-04 . - Asian Institute of Technology. Dissertation ; no. EV-11-04 .
A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development
Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2011
The estrogenic activities of sesame lignans and their metabolites were investigated by using a viability assay and estrogen response elements (ERE) rep01ier system on the different levels of estrogen in cultured media. Hormone-dependent human breast cancer T47D cells and the cells stably transfected with ERE-luciferase reporter system T47DKBluc cells were utilized. Depending on levels of E2, sesame lignans and their metabolites exhibited different effects on the viability of T47D-KBluc cells. In the absence of estrogen, the trend of induction of cell growth was observed, whereas in the presence of estrogen, cytotoxic effect was evidenced. The ERE luciferase rep01ier assay in T47DKB1uc cells shows that, sesame lignans and their metabolites induced ERE activation. Among tested compounds, sesamol possessed the highest ERE activation prope1iy while ED showed no effect. All tested compounds exhibited lower levels of ERE activation than that of estradiol (E2) activation, suggesting that the tested compounds possess weak estrogenic effect. The antiestogen ICI 182 780 significantly decreased ERE activation induced by the te
Seseme
Estrogen
Breast--Cancer
Estrogenic activities of sesame lignans and their metabolites : interactive effect with cadmium in human breast cancer cell lines - Pathum Thani, Thailand : Asian Institute of Technology, 2011 - 91 leaves : ill. (some col.) - Dissertation ; no. EV-11-04 . - Asian Institute of Technology. Dissertation ; no. EV-11-04 .
A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development
Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2011
The estrogenic activities of sesame lignans and their metabolites were investigated by using a viability assay and estrogen response elements (ERE) rep01ier system on the different levels of estrogen in cultured media. Hormone-dependent human breast cancer T47D cells and the cells stably transfected with ERE-luciferase reporter system T47DKBluc cells were utilized. Depending on levels of E2, sesame lignans and their metabolites exhibited different effects on the viability of T47D-KBluc cells. In the absence of estrogen, the trend of induction of cell growth was observed, whereas in the presence of estrogen, cytotoxic effect was evidenced. The ERE luciferase rep01ier assay in T47DKB1uc cells shows that, sesame lignans and their metabolites induced ERE activation. Among tested compounds, sesamol possessed the highest ERE activation prope1iy while ED showed no effect. All tested compounds exhibited lower levels of ERE activation than that of estradiol (E2) activation, suggesting that the tested compounds possess weak estrogenic effect. The antiestogen ICI 182 780 significantly decreased ERE activation induced by the te
Seseme
Estrogen
Breast--Cancer