Cloning of fungal chitin deacetylase gene into bacterial Escherichia coli (Record no. 18113)

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005 - DATE AND TIME OF LATEST TRANSACTION
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008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 020215s2001 th uzm rtt 00| a1eng d
035 ## - SYSTEM CONTROL NUMBER
System control number .b1184596x
099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number AIT Thesis no. BP-01-05
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Nguyen Hong Nhung
245 10 - TITLE STATEMENT
Title Cloning of fungal chitin deacetylase gene into bacterial Escherichia coli
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Bangkok :
Name of publisher, distributor, etc. Asian Institute of Technology,
Date of publication, distribution, etc. 2001
300 ## - PHYSICAL DESCRIPTION
Extent 40 leaves
490 1# - SERIES STATEMENT
Series statement Thesis ;
Volume/sequential designation no. BP-01-05
500 ## - GENERAL NOTE
General note A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Asian Institute of Technology,2001
520 ## - SUMMARY, ETC.
Summary, etc. Chitin deacetylase(CDA)gene from Colletotrichum lindemuthianum UPS9 has been used in this study. The gene cloned into shuttle vector pPIC9K has been used as a source of CDA gene throughout the study. Large quantity of gene was obtained allowing its multiplication in component E.coli TOPlOF'. QIAGEN plasmid midi Kit was used for the extraction and purification of the DNA. The gene was integrated into a another shuttle vector pPIC3.5K in its multiple cloning site. The plasmid vector pPIC3.5Kb is 9.0 kb long plasmid earring ampicillin and kanamycin resistant markers. It can be multiplied in E.coli as well as in Picia pastoris. In E.coli, it remains extrachromosomally and multiplies independently. It remains as a low copy number plasmid. On the other hand , in Pichia pastoris, it intergrates into genome via homologous recombination and results in stable transfmmation. In this research work, CDA gene has been intergrated in between EcoRI and Natl sites of multiple cloning sites. Recombinant plasmid was transformed into competent E.coli TOPIOF' intermediate host by calcium chloride method in order to obtain the plasmid in large quantity. The integration of CDA gene was confimed by restriction digestion analysis followed by gel electrophoretic migration and size comparison.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Chitin
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Escherichia coli
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Stevens, Willem Frans,
Relator term Chairperson
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Suwalee Chandrkrachang,
Relator term Examination committee
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sunee Nitisinprasert,
Relator term Examination committee
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Macaranas, Julie M.,
Relator term Examination committee
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Swedish International Development Cooperation Agency (Sida),
Relator term Scholarship donor
810 2# - SERIES ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Asian Institute of Technology.
Title of a work Thesis ;
Volume/sequential designation no. BP-01-05
856 ## - ELECTRONIC LOCATION AND ACCESS
Materials specified Full-Text
Uniform Resource Identifier <a href="http://203.159.5.9/ait-thesis/detail.php?q=B07827">http://203.159.5.9/ait-thesis/detail.php?q=B07827</a>
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a 240329
998 ## - LOCAL CONTROL INFORMATION (RLIN)
Operator's initials, OID (RLIN) 0
Cataloger's initials, CIN (RLIN) 020215
First date, FD (RLIN) m
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945 ## - LOCAL PROCESSING INFORMATION (OCLC)
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945 ## - LOCAL PROCESSING INFORMATION (OCLC)
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type 22-AIT Thesis (Replacement)
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type 40-Archives
909 ## - LOCAL ITEMS USED
Barcode Barcode : 30050120683056
CREATED CREATED : 2013-06-09
RECORD Id RECORD # : i12740019
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Barcode Barcode : 30050120350888
CREATED CREATED : 2016-10-06
RECORD Id RECORD # : i13029605
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Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Cost, normal purchase price Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
      Available for Loans Asian Institute of Technology Library Asian Institute of Technology Library AIT Publications 17/08/2026 50.00   AIT Thesis no. BP-01-05 30050120683056 17/08/2026 3 17/08/2026 22-AIT Thesis (Replacement)
      Available for Loans Asian Institute of Technology Library Asian Institute of Technology Library Archives 17/08/2026     AIT Thesis no. BP-01-05 30050120350888 17/08/2026   17/08/2026 40-Archives
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