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Cloning of fungal chitin deacetylase gene into yeast Pichia pastoris (Record no. 18114)

MARC details
000 -LEADER
fixed length control field	03206nas\|a2200445 i 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field	20260817172937.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	020717s2000 th u m rtt 00\| a1eng d
035 ## - SYSTEM CONTROL NUMBER
System control number	.b11861435
099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number	AIT Thesis no. BP-00-10
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name	Shrestha, Binesh
245 10 - TITLE STATEMENT
Title	Cloning of fungal chitin deacetylase gene into yeast Pichia pastoris
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc.	Bangkok :
Name of publisher, distributor, etc.	Asian Institute of Technology,
Date of publication, distribution, etc.	2000
300 ## - PHYSICAL DESCRIPTION
Extent	82, [2] leaves
490 1# - SERIES STATEMENT
Series statement	Thesis ;
Volume/sequential designation	no. BP-00-10
500 ## - GENERAL NOTE
General note	A thesis submitted in partical fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
502 ## - DISSERTATION NOTE
Dissertation note	Thesis (M.Sc.) - Asian Institute of Technology, 2000
520 ## - SUMMARY, ETC.
Summary, etc.	Chitin deacetylase (CDA) gene has been amplified from Colletotrichum lindemuthianum UPS9 DNA and cloned into one of the E.coli-Pichia pastoris shuttle vector (pPlC9K). The sequence analysis of cloned gene showed 98% homology with that of C. /indemuthianum ATCC56676. The gene was cloned into pPIC9K in frame with the asecretion signal factor present in the vector. Furthermore, the gene was transformed into Pichia pastoris expression host in order to obtain overexpressing strains. Two strains of Pichia namely GS115 (fast methanol utilizing, Mut+) and KM71 (slow methanol utilizing, Mut5 ) were selected as a host for the exrpession of CDA gene. Pichia transformants were selected on the basis of Histidine synthesizing phenotype and were screened for transformants carrying multi copy inserts of CDA gene on the basis of degree of resistance to G418 (Geneticine ). Transformants carrying resistance upto 3 mg/ml of G418 were screened out which might carry several copies of cloned CDA gene. The expression of cloned CDA gene in Pichia transformants were studied by analysing culture supernatant on SOS-PAGE for excreted protein expression.
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element	Molecular cloning
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element	Chitin
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name	Stevens, Willem Frans,
Relator term	Chairperson
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name	Hegarat, Francoise Le,
Relator term	Co-Chairperson
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name	Suwalee Chandrkrachang,
Relator term	Examination committee
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name	Pakorn Nuchnoi,
Relator term	Examiantion Committee
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name	Sunee Nitisinprasert,
Relator term	Examination committee
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element	H.M. the King of Thailand,
Relator term	Scholarship donor
810 2# - SERIES ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element	Asian Institute of Technology.
Title of a work	Thesis ;
Volume/sequential designation	no. BP-00-10
856 ## - ELECTRONIC LOCATION AND ACCESS
Materials specified	Full-Text
Uniform Resource Identifier	<a href="http://203.159.5.9/ait-thesis/detail.php?q=B06980">http://203.159.5.9/ait-thesis/detail.php?q=B06980</a>
907 ## - LOCAL DATA ELEMENT G, LDG (RLIN)
a	.b11861435
b	mnait
c	u
902 ## - LOCAL DATA ELEMENT B, LDB (RLIN)
a	240402
998 ## - LOCAL CONTROL INFORMATION (RLIN)
Operator's initials, OID (RLIN)	0
Cataloger's initials, CIN (RLIN)	020717
First date, FD (RLIN)	m
--	a
--	u
--	0
945 ## - LOCAL PROCESSING INFORMATION (OCLC)
l	mnait
945 ## - LOCAL PROCESSING INFORMATION (OCLC)
l	mnait
945 ## - LOCAL PROCESSING INFORMATION (OCLC)
l	mnarc
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	20-AIT Publication
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	40-Archives
909 ## - LOCAL ITEMS USED
Barcode	Barcode : 30050120682876
CREATED	CREATED : 2001-02-17
RECORD Id	RECORD # : i12291018
LPATRON	LPATRON : 1015270
LCHKIN	LCHKIN : 2003-02-21
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909 ## - LOCAL ITEMS USED
Barcode	Barcode : 30050120682850
CREATED	CREATED : 2001-02-17
RECORD Id	RECORD # : i1229102x
LPATRON	LPATRON : 1016802
LCHKIN	LCHKIN : 2004-02-29
RENEWALS	# RENEWALS : 0
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909 ## - LOCAL ITEMS USED
Barcode	Barcode : 30050120350821
CREATED	CREATED : 2016-10-06
RECORD Id	RECORD # : i1302968x
LPATRON	LPATRON : 0
LCHKIN	LCHKIN : -
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Holdings
Not for loan	Home library	Current library	Shelving location	Date acquired	Cost, normal purchase price	Full call number	Barcode	Date last seen	Copy number	Price effective from	Koha item type
Available for Loans	Asian Institute of Technology Library	Asian Institute of Technology Library	AIT Publications	17/08/2026	50.00	AIT Thesis no. BP-00-10	30050120682876	17/08/2026	1	17/08/2026	20-AIT Publication
Available for Loans	Asian Institute of Technology Library	Asian Institute of Technology Library	AIT Publications	17/08/2026	50.00	AIT Thesis no. BP-00-10	30050120682850	17/08/2026	2	17/08/2026	20-AIT Publication
Available for Loans	Asian Institute of Technology Library	Asian Institute of Technology Library	Archives	17/08/2026		AIT Thesis no. BP-00-10	30050120350821	17/08/2026		17/08/2026	40-Archives

Cloning of fungal chitin deacetylase gene into yeast Pichia pastoris (Record no. 18114)

AIT

Asian Institute of Technology Library