AI SearchCloning of the chitin deacetylase gene from the fungus Mucor rouxii into the yeast Pichia pastoris (Record no. 18116)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03690nas|a2200433 i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260817172938.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 020717s2002 th u m rtt 00| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b11861393 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no. BP-02-01 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dar, Shabir Ahmad |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning of the chitin deacetylase gene from the fungus Mucor rouxii into the yeast Pichia pastoris |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Bangkok : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2002 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 61, [12] leaves |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. BP-02-01 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirement for the degree of Master of Science, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M. Sc.) - Asian Institute of Technology, 2002 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Chitin, which occurs in the exoskeleton of arthropods and in the cell wall of fungi, is an abundant renewable natural resource. Chitosan, the deacetylated form of chitin has a broad variety of industrial and biomedical applications. It is traditionally produced by a harsh thermochemical procedure that is environmentally unsafe and not easily controlled. The use of chitin deacetylases offers the possibility of an alternative procedure to the chemical process for the deacetylation of chitin. Molecular cloning of CDA gene into a suitable host is therefore explored to achieve efficient production of the enzyme. This work aimed at the cloning of CDA gene from Mucor rouxii and the constructing of CDA-overexpressing strains of Pichia pastoris. Chitin deacetylase (CDA) gene after amplification from Mucor rouxii ATCC 24905 was cloned into two of the E.coli-Pichia pastoris shuttle vectors, pPIC3.5K and pPIC9K. In case of pPIC9K the gene was cloned in phase with the a-secretion signal factor present in the parent vector for the secreted expression of CDA. The gene was put in both vectors under the transcriptional control of Pichia pastoris alcohol oxidase gene promoter. The gene was transformed into Pichia pastoris GS115 (methanol utilization fast, Mut) to obtain overexpressing strains. Pichia transformants were selected on histidine deficient medium and screened for their ability to use methanol as a sole source of carbon. The selected clones were screened for transformants resistant to G418 (Geneticin). Integration of the gene in Pichia genome was confirmed through PCR analysis of the transformants. The intracellular and secreted expression of the cloned CDA gene in Pichia transformants was analyzed through SDSp AGE analysis. |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Molecular cloning |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chitin |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stevens, Willem Frans, |
| Relator term | Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hegarat, Francoise Le, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Suwalee Chandrkrachang, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sunee Nitisinprasert, |
| Relator term | Examination Committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Macaranas, Julie, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | The Royal Netherlands Government, |
| Relator term | Scholarship Donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. BP-02-01 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B08077">http://203.159.5.9/ait-thesis/detail.php?q=B08077</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b11861393 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240402 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 020717 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 22-AIT Thesis (Replacement) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120682959 |
| CREATED | CREATED : 2013-06-09 |
| RECORD Id | RECORD # : i12739972 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120350946 |
| CREATED | CREATED : 2016-10-06 |
| RECORD Id | RECORD # : i13029502 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 17/08/2026 | 50.00 | AIT Thesis no. BP-02-01 | 30050120682959 | 17/08/2026 | 3 | 17/08/2026 | 22-AIT Thesis (Replacement) | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 17/08/2026 | AIT Thesis no. BP-02-01 | 30050120350946 | 17/08/2026 | 17/08/2026 | 40-Archives |