AI SearchCopper resistant mechanism in Agrobacterium tumefaciens NTL4 (Record no. 23431)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03654nas|a2200409 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818085159.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 060911s2006 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b11992669 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.EV-06-1 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Anchalee Charoenwuttitam |
| 245 10 - TITLE STATEMENT | |
| Title | Copper resistant mechanism in Agrobacterium tumefaciens NTL4 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2006 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 86 leaves : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. EV-06-1 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science Inter-University Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology, 2006 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Agrohacterum tumefaciens possesses multiple system to ensure proper copper handling under varying environmental condition. Searching A. tumefaciens C58 genome revealed that the bacteria had three loci (two on circular and one on linear chromosome) contained genes involved in copper resistance. Here, three genes, namely cueR (Atu0938), copA (Atu0937) and CueO (Atu3991) from two loci were functionally evaluated in term of copper resistance. CueR is transcriptional regulator in a MerR family while CopA and CueO are copper transporting P-type APpase and multicopper oxidase, respectively. A. tumefaciens cueR, copA and cueO. Mutants were constructed by gene knockout using pKNOCK system. cueO mutant was sensitive to Cu (II) and Cd (II) as tested by inhibition zone assays however all the MIC values against Cu (II) in all the mutant was reduced comparing to the wild type's level. The altered phenotypes could be restored by a plasmidborne expression of individual gene. Northern blot analysis revealed that the expression of cueO could be induced only by Cu (II) in an CueR-independent manner. Promoter analysis using lacZ fusion revealed that copA expression was controlled by CueR and could be hypothesized that when A. tumefaciens exposes to increase level of copper (as well as Ag) ions. Two transcription regulators, CueR and the unknown regulator, respond to this change by promote transcription of copA encoding efflux protein and cueO encode multicopper oxidase. High expression of these two protein makes A. tumefaciens more resistant to copper |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Copper |
| General subdivision | Environmental aspects |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Paiboon Vattanaviboon, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Skorn Mongkolsuk, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Preeda Pakpian, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Chulabhom Research Institute I Mahidol University I Asian Institute of Technology (CRI-MU-AIT), |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. EV-06-1 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B10590">http://203.159.5.9/ait-thesis/detail.php?q=B10590</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b11992669 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240401 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 060911 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120737217 |
| CREATED | CREATED : 2005-04-14 |
| RECORD Id | RECORD # : i12495712 |
| LPATRON | LPATRON : 1020210 |
| LCHKIN | LCHKIN : 2008-04-02 |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 2 |
| -- | TOT CHKOUT : 5 |
| -- | TOT RENEW : 0 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120737183 |
| CREATED | CREATED : 2005-04-14 |
| RECORD Id | RECORD # : i12495724 |
| LPATRON | LPATRON : 1018265 |
| LCHKIN | LCHKIN : 2006-01-11 |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 2 |
| -- | TOT RENEW : 2 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050160050422 |
| CREATED | CREATED : 2016-05-25 |
| RECORD Id | RECORD # : i12991697 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-06-1 | 30050120737217 | 18/08/2026 | 1 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-06-1 | 30050120737183 | 18/08/2026 | 2 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.EV-06-1 | 30050160050422 | 18/08/2026 | 18/08/2026 | 40-Archives |