AI SearchDevelopment of an electrochemical device capable of loop mediated isothermal amplification (LAMP) to detect hepatitis B (Record no. 29126)
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| 000 -LEADER | |
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| fixed length control field | 04753nas a2200457 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818090800.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190826s2017 th uu m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12310037 |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.FB-17-02 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Jayanath, Nileththi Yasendra |
| 245 10 - TITLE STATEMENT | |
| Title | Development of an electrochemical device capable of loop mediated isothermal amplification (LAMP) to detect hepatitis B |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 93 leaves : |
| Other physical details | ill. + |
| Accompanying material | 1 online resource |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no.FB-17-02 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M. Sc.) - Asian Institute of Technology, 2017 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A sensitive, simple and inexpensive technique for point - of - care (POC) diagnosis of hepatitis B (HBV) is highly desirable majorly due to the lack of advanced medical and diagnostic facilities in some endemic areas . The objective of this study was to develop a low - cost and simple device based on Loop - mediated isothermal amplification (LAMP) of DNA and electrochemical measurement for rapid detection of HBV. The electrochemical mediato r methylene blue (MB) was intercalated into the LAMP amplicons and square wave voltammetry (SWV) signals were processed by a hand - held potentiostat. SWV response of MB was studied with different concentrations. LAMP was optimized for temperature and MB con centrations for real time monitoring. Quantification curves were developed followed by testing of unknowns, using conventional and sigmoidal model fitting (4 parametric) approaches whereas both peak height ratio (PHR) and amplification times were used as t hresholds. Sensitivity and specificity were assessed followed by testing human plasma without DNA extraction. A positive linear relationship of peak currents of SWV vs concentrations of MB was observed with significantly higher currents at higher temperat ure. Incubation temperature of 63 o C and 25 o M of MB were the optimized condition for LAMP based on reduction of PHRs. Linear relationships between logarithmic concentrations of HBV DNA vs amplification time and PHRs depending on the thresholds were obtain ed with different coefficient of determination (R 2 ) value. Peak height thresholds of 0.6 and time threshold of 22 min were found to be more suited in conventional approach while the former varied to 0.7 and latter remined same in model fitting approach, ba sed on R 2 . Concentrations of the unknowns were quantified successfully to the logarithmic concentrations of DNA. The a pproach was found to be specific for HBV as no PHR reduction for human genomic DNA and HBV DNA up to fg/ o l was detected . Human plasma coul d be used directly as the temp late after a pre - heat treatment of either subsequent heating for 5 min at 95 o C and 100 o C or 125 o C for 10 min whereas the centrifugation step of the former increased the LAMP efficiency. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Hepatitis B |
| General subdivision | Prevention |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Loc Thai Nguyen, |
| Relator term | Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Anal, Anil Kumar, |
| Relator term | Examination Committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sadiq, Muhammad Bilal, |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology Fellowship, |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. no.FB-17-02 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B02861">http://203.159.5.9/ait-thesis/detail.php?q=B02861</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12310037 |
| b | mnait |
| c | m |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240422 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 190826 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | m |
| -- | 0 |
| 962 ## - | |
| -- | 000:001:PDF:b1231003:004334:0:0:0:0:0:0 |
| -- | Click to view Abstract |
| -- | n |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 61-CD-ROM |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120894471 |
| CREATED | CREATED : 2019-05-23 |
| RECORD Id | RECORD # : i13188458 |
| LPATRON | LPATRON : 0 |
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| Barcode | Barcode : - |
| CREATED | CREATED : 2019-05-23 |
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| Barcode | Barcode : 30050120955579 |
| CREATED | CREATED : 2019-08-26 |
| RECORD Id | RECORD # : i13213751 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120955546 |
| CREATED | CREATED : 2019-08-26 |
| RECORD Id | RECORD # : i13213763 |
| LPATRON | LPATRON : 0 |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type | Cost, normal purchase price |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.FB-17-02 | 30050120894471 | 18/08/2026 | 1 | 18/08/2026 | 40-Archives | |||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.FB-17-02 | 18/08/2026 | 18/08/2026 | 61-CD-ROM | |||||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | AIT Thesis no.FB-17-02 | 30050120955579 | 18/08/2026 | 1 | 18/08/2026 | 20-AIT Publication | 50.00 | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | AIT Thesis no.FB-17-02 | 30050120955546 | 18/08/2026 | 2 | 18/08/2026 | 20-AIT Publication | 50.00 |