AI SearchDevelopment of novel epitope-imprinted polydopamine (PDA) interface for electrochemical detection of gluten in foods (Record no. 29421)
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| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818090900.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
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| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12373539 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.FB-21-03 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Corpuz, Angelica Revibes |
| 245 10 - TITLE STATEMENT | |
| Title | Development of novel epitope-imprinted polydopamine (PDA) interface for electrochemical detection of gluten in foods |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 156 leaves : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no.FB-21-03 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M. Eng.) - Asian Institute of Technology, 2021 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The drawbacks of the conventional gluten detection techniques have put biosensors in the spotlight. They can be functionalized to become highly sensitive, selective, simple and cheap detection platforms for gluten-free food testing and verification. In this study, nanomaterial amplification and PDA-based epitope imprinting were applied for voltammetric detection of gliadin, a known pathogenic component of gluten. Critical synthesis and sensing conditions were optimized based on current response while voltammetric response, SEM, Raman spectroscopy and FTIR confirmed the modification. The electrochemical synthesis proved to be effective in depositing conductive RGO film and then imprinted polymeric film on bare SPCE. It was crucial to optimize the thickness of the RGO (10 CV scans) and polymeric (20 CV scans) films, template concentration (0.5 g/L), pre-polymerization time (30 mins), elution time (30 mins), rebinding pH (6.5) and time (10 mins) for a highly sensitive and selective interface. The sensor was able to detect native gliadins in buffered matrix in the linear range of 0.5-15ppm (LOD 0.69, LOQ 2.29) with high selectivity for PQQPFPQQ template (IF=8.68) and gliadin (IF=3.14) and can discriminate against OVA (Ý=0.15), FA (Ý=0.25) and casein (Ý=0.39). It is stable at room temperature for 2 weeks (7.52% RSD), reproducible (2.97% RSD) and reusable (1.64% RSD) once. Negligible matrix effects and reproducible measurements were found in rice flour matrix with higher recovery rates (101.96% and 99.19% for 1.15 and 10 ppm, respectively) than that of ELISA test kit II of Morinaga Institute (82.61% and 83.50%). This suggested the accessibility of epitope on surface of native gliadin and the feasibility of using an acidic template for PDA-based imprinting. The developed technique was able to surpass performance of more sophisticated EG-FET biosensor and a label-free immunoassay demonstrating its robustness. Evaluation of applicability to other toxic prolamins, hydrolyzed or heat-processed gluten sources and other food matrixes were recommended. Finally, certification of the spiking standard material to attest its actual concentration and completion of three data points for proper linear correlation with ELISA is proposed. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Proteins |
| General subdivision | Analysis |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Electrochemistry |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Loc Thai Nguyen, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Suwussa Bamrungsap, |
| Relator term | Co-Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Anal, Anil Kumar, |
| Relator term | Examination Committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salin, Krishna R., |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Development Bank-Japan Scholarship Program (ADB-JSP), |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no.FB-21-03 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B14882">http://203.159.5.9/ait-thesis/detail.php?q=B14882</a> |
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| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
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| Cataloger's initials, CIN (RLIN) | 211006 |
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| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
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| Koha item type | 67-Electronic Resource |
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| Koha item type | 20-AIT Publication |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120885461 |
| CREATED | CREATED : 2021-04-10 |
| RECORD Id | RECORD # : i13351679 |
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| CREATED | CREATED : 2021-04-10 |
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| Barcode | Barcode : 30050121023914 |
| CREATED | CREATED : 2022-12-16 |
| RECORD Id | RECORD # : i13420173 |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type | Cost, normal purchase price |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.FB-21-03 | 30050120885461 | 18/08/2026 | 1 | 18/08/2026 | 40-Archives | |||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.FB-21-03 | 18/08/2026 | 18/08/2026 | 67-Electronic Resource | |||||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | AIT Thesis no.FB-21-03 | 30050121023914 | 18/08/2026 | 1 | 18/08/2026 | 20-AIT Publication | 50.00 |