AI SearchDevelopment of robust static in vitro gut model to evaluate the behavior of probiotics with dietary fiber (Record no. 29492)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03901nas a2200361 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818090916.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211006s2021 th uu m rtt 0| a1eng |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12373989 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.FB-21-07 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Gurung, Anurag |
| 245 10 - TITLE STATEMENT | |
| Title | Development of robust static in vitro gut model to evaluate the behavior of probiotics with dietary fiber |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 181 leaves : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no.FB-21-07 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M. Sc.) - Asian Institute of Technology, 2021 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The main aim of this research was to design and fabricate an in vitro gastrointestinal model to study the effects of dietary fiber on the behavior of probiotics in simulated gastrointestinal conditions. In this research, the effect of inulin on the viability and the ability of probiotics to produce short chain fatty acids (SCFAs) during simulated gastrointestinal digestion was studied. Simulated digestion in the in vitro gut model was separated into two batch-type processes which were the gastric digestion and colon fermentation phases. The probiotic bacteria strains, Streptococcus thermophilus and Lactobacillus bulgaricus, were digested in the in vitro gut model in the presence of inulin. For this purpose, yoghurts with inulin at 0.5 %, 1 % and 2 % (w/v) concentrations were prepared using the two specified probiotics. Physiochemical analyses of yoghurt samples found that the addition of inulin had positive effects on its viscosity and probiotic cell count. However, the effects were not found to differ significantly from that of control yoghurt. During in vitro gastric digestion, all types of yoghurts experienced reduction in their total cell log CFU. Yoghurt with 2 % (w/v) inulin had the highest number of viable bacterial cells (3.72 log CFU ± 0.2) at the end of gastric digestion and control yoghurt had the lowest total cell log CFU (2.89 log CFU ± 0.05). Simulated in vitro colonic fermentation of yoghurt samples also resulted in gradual reduction of viable bacterial numbers but not to the same extent as observed during the gastric digestion. Yoghurt with 0.2 % (w/v) inulin had the highest viable bacterial cell numbers (6.63 log CFU ± 0.15) whilst control yoghurt had the lowest (4.6 log CFU ± 0.17). The highest value of SCFA released during in vitro colonic fermentation was recorded for yoghurt with 0.2 % (w/v) inulin (122.5 æmol ± 1.4). The value of SCFA released during simulated colonic fermentation increased with increasing incubation time (h) and was higher for yoghurts with higher concentration of inulin. Therefore, the viability and metabolic activity of bacteria was observed to have been affected positively by the presence of inulin. The static in vitro gut model was also able to successfully simulate the gastrointestinal conditions. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Functional foods |
| General subdivision | Development |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Probiotics |
| General subdivision | Health aspects |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Anal, Anil Kumar, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Loc Thai Nguyen, |
| Relator term | Examination committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bhujel, Ram C., |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology Fellowship, |
| Relator term | Scholarship Donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no.FB-21-07 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B14886">http://203.159.5.9/ait-thesis/detail.php?q=B14886</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12373989 |
| b | mnarc |
| c | m |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240418 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 211006 |
| First date, FD (RLIN) | m |
| -- | b |
| -- | m |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 67-Electronic Resource |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : - |
| CREATED | CREATED : 2021-06-10 |
| RECORD Id | RECORD # : i13352532 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.FB-21-07 | 18/08/2026 | 1 | 18/08/2026 | 67-Electronic Resource |