AI SearchEffects of cadmium on DNA damage and expression of repair gene (Record no. 35038)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04586nas a2200433 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818095220.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 050929s2005 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b11971757 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.EV-05-31 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Gotovdorj, Tuvshinjargal |
| 245 10 - TITLE STATEMENT | |
| Title | Effects of cadmium on DNA damage and expression of repair gene |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2005 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 64 leaves |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. EV-05-31 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology, 2005 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science Inter-University Program on Environmental Toxicology, Technology and Management |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Cadmium is a highly toxic heavy metal; however it has been used worldwide. It has been classified a human carcinogen by IARC. Cadmium is genotoxic substance due to it can cause damage to DNA and chromosome. In this study, the effects of cadmium treatment on DNA damage and repair were studied in the human lymphocytes. The dose-response relationship between cadmium treatment and DNA strand breaks was studied in the human lymphocytes treated with 5, 10, 25, 50 and 100M cadmium. Using comet assay, the effects of cadmium treatment at various concentrations caused a significant induction of DNA strand breaks in a dose-dependent manner up to 50M and slightly increased further at 100M in the human lymphocytes. A The time-course of cadmium treatment on an induction of DNA strand breaks was carried out in lymphocytes treated with cadmium for 2, 4 and 24 hours. The effects of cadmium on the DNA strand breaks were also observed in the timedependent manner in the human lymphocytes. The induction of DNA strand breaks indicated a percentage of increase from control was highest at 4 hours following cadmium treatment at all test concentrations, and then dramatically decreased towards 24 hours of treatment. Cadmium treatment at 50M for 4 hours was then selected as the optimum dose and time of cadmium on the DNA damage. The effect of cadmium treatment on the oxidized base DNA damage was studied in human lymphocytes treated at 50M cadmium for 4 hours by comet assay with formamidopyrimidine-DNA-glycosylase (Fpg). The results showed that cadmium significant increased the Fpg-sensitive sites in the human lymphocytes by 1.4-fold (P<0.05), compared to control. Cadmium can cause oxidized base DNA damage. The alterations of repair gene (hOGG1) and metallothionein (MT) gene expression were studied in human lymphocytes treated with 50M cadmium for 4 hours by Reverse-Transcriptase and Polymerase Chain Reaction (RT-PCR). Cadmium induced MT mRNA expression by 1.62-fold (P<0.01) from control. In contrast, cadmium treatment resulted in decrease in the levels of hOGG1 by 1.67-fold (P<0.01), compared to control. In conclusion, the present study demonstrated that cadmium significantly increased DNA strand breaks in human lymphocytes in a dose and time-dependent. In addition, a significant increase in oxidized base damage indicated as Fpg-sensitive base was in accordance with the reduction of repair gene (hOGG1) in human lymphocytes treated with cadmium. Therefore, cadmium can cause genotoxic to human lymphocytes possibly by induction of oxidative DNA damage and of DNA repair inhibition. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA damage |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA repair |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cadmium |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Panida Navasumrit, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Preeda Pakpian, |
| Relator term | Examination Committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Piyajit Watcharasit, |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Her Majesty The Queen of Thailand |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. EV-05-31 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B09401">http://203.159.5.9/ait-thesis/detail.php?q=B09401</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b11971757 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240329 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 050929 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050004612114 |
| CREATED | CREATED : 2004-02-05 |
| RECORD Id | RECORD # : i1246126x |
| LPATRON | LPATRON : 1017138 |
| LCHKIN | LCHKIN : 2004-09-12 |
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| Barcode | Barcode : 30050120737357 |
| CREATED | CREATED : 2004-02-05 |
| RECORD Id | RECORD # : i12461271 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050160050505 |
| CREATED | CREATED : 2016-05-25 |
| RECORD Id | RECORD # : i12991533 |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-05-31 | 30050004612114 | 18/08/2026 | 1 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-05-31 | 30050120737357 | 18/08/2026 | 2 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.EV-05-31 | 30050160050505 | 18/08/2026 | 18/08/2026 | 40-Archives |