AI SearchExtracellular expression of fungal chitin deacetylase gene in the yeast Pichia pastoris (Record no. 42658)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03459nas|a2200409 i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818113221.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 020208s2001 th uzm rtt 00| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b11844590 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no. BP-01-03 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dinuriah, Imastini |
| 245 10 - TITLE STATEMENT | |
| Title | Extracellular expression of fungal chitin deacetylase gene in the yeast Pichia pastoris |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Bangkok : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2001 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 47 leaves |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. BP-01-03 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology, 2001 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The use of chitin deacetylase enzyme (CDA) for converting chitin into chitosan catches the attention of many scientists. CDA genes from various sources of organisms have been cloned into fast growing bacteria or yeasts to increase CDA production. Following the success on cloning the CDA gene from Colletotrichum lindemuthianum UPS9 into the yeast Pichia pastoris strain GS115 (Mut) and KM71 (Muts) via plasmid shuttle vector pPIC9K done by Shrestha (2000), the CDA gene expression of his recombinant clones was investigated. Selection of the best CDA producer among the recombinant clones in the expression medium was carried out based on the CDA activity of each clone on a partially deacetylated chitin substrate (PDC, 65% DD 200 mesh). CDA activity was analyzed by using Gas-Liquid Chromatography based on the release of acetic acid during the enzyme assay. Clone Cl-6 was selected as the best CDA producer out of 63 clones tested. The effect of several factors on the production and characteristics of the CDA of the best clone was also studied. The CDA production was better in buffered minimal methanol medium than in non-buffered minimal methanol medium. Enzyme levels were inhibited rather than enhanced if chitin with different levels of deacetylation was included in the expression medium. The optimum condition for CDA assay was at 50°C and pH 5.8. It was found that the CDA of clone Cl-6 was not inhibited by acetate during the enzyme assay. The test on thermostability showed that the CDA activity remaining was more than 90% even after exposing the CDA to 50°C for one hour before enzyme assay but not for more than 3 hours |
| 650 1# - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chitin |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pichia pastoris |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stevens, Willem Frans, |
| Relator term | Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Suwalee Chandrkrachang, |
| Relator term | Examination committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sunee Nithisinprasert, |
| Relator term | Examination committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Macaranas, Julie, |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | DUE Project Batch II, LPIU - UNSOED, Indonesia, |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. BP-01-03 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B07823">http://203.159.5.9/ait-thesis/detail.php?q=B07823</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b11844590 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240329 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 020208 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120683098 |
| CREATED | CREATED : 2013-06-09 |
| RECORD Id | RECORD # : i12740020 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120350870 |
| CREATED | CREATED : 2016-10-06 |
| RECORD Id | RECORD # : i13029630 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no. BP-01-03 | 30050120683098 | 18/08/2026 | 3 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no. BP-01-03 | 30050120350870 | 18/08/2026 | 18/08/2026 | 40-Archives |