AI SearchFunctional and expression analyses of cop operon required for copper resistance in Agrobacterium tumefaciens (Record no. 47748)
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| 000 -LEADER | |
|---|---|
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| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818114608.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 071114s2007 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b1191340x |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.EV-07-48 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sirikan Nawapan |
| 245 10 - TITLE STATEMENT | |
| Title | Functional and expression analyses of cop operon required for copper resistance in Agrobacterium tumefaciens |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2007 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 57 leaves : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. EV-07-48 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2007 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The cop operon of Agrobacterium tumefaciens involving in copper resistance is consisted of three genes that encode CPx-type copper ATPase, CopA, a repressor, CopR and a copper chaperone, CopZ. The expression of cop operon is induced by the presence of copper and silver thereby protecting A. trmrefaciens from particular metal ion toxicity. Primer extension analysis defined the consensus sequence 5'CTTCCCATnATGGGAAG3' as the repressor binding clement. Using in vivo copARZ promoter analysis, it was demonstrated that copA expression is regulated by copR while CopZ copper chaperone played no role on the function of CopR to sensing copper. Gene disruption of copA and copZ by using the suicide plasmid pKNOCK caused reduction of bacterial ability to cope with copper and silver toxicity. Unfortunately, such reduction could not be complemented by a trans expression of copA or copZ respectively resulting from the polar effect from inactivation of one gene on the expression of other genes in the operon. Taken together, the results suggest that under copper-limiting condition CopR binds to the copARZ promoter and represses the expression of the operon. In high copper, CopR is activated to an active form of transcription activator thereby, enhancing copARZ transcript. A copper chaperone, CopZ then binds excess free Cu(I) and transfers to CopA copper export to detoxify copper |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Agrobacterium tumefaciens |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Copper |
| General subdivision | Toxicity testing |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Paiboon Vattanaviboon., |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Skorn Mongkolsuk, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Preeda Pakpian, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Chulabhom Research Institute, |
| Relator term | Scholarship Donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Mahidol University, |
| Relator term | Scholarship Donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology, |
| Relator term | Scholarship Donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. EV-07-48 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href=" http://203.159.5.9/ait-thesis/detail.php?q=B11006"> http://203.159.5.9/ait-thesis/detail.php?q=B11006</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b1191340x |
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| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240411 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 071114 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120742985 |
| CREATED | CREATED : 2002-09-24 |
| RECORD Id | RECORD # : i1237734x |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120742993 |
| CREATED | CREATED : 2006-06-17 |
| RECORD Id | RECORD # : i12545806 |
| LPATRON | LPATRON : 1019935 |
| LCHKIN | LCHKIN : 2007-02-28 |
| RENEWALS | # RENEWALS : 0 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050160049440 |
| CREATED | CREATED : 2016-05-31 |
| RECORD Id | RECORD # : i13005200 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-07-48 | 30050120742985 | 18/08/2026 | 1 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | 50.00 | AIT Thesis no.EV-07-48 | 30050120742993 | 18/08/2026 | 2 | 18/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Thesis no.EV-07-48 | 30050160049440 | 18/08/2026 | 18/08/2026 | 40-Archives |