AI SearchAnalyzing biodegradability of low-density polyethylene by microorganisms from dumping site and enhancement of their biodegradation potential (Record no. 5395)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03973nas a2200385 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260817162541.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250911s20249999th u ms t 000 eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12464089 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Diss. no.FB-24-01 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Pornya Khampratueng |
| 245 10 - TITLE STATEMENT | |
| Title | Analyzing biodegradability of low-density polyethylene by microorganisms from dumping site and enhancement of their biodegradation potential |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2024 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 183 leaves : |
| Other physical details | ill.+ |
| Accompanying material | 1 online resource |
| 490 1# - SERIES STATEMENT | |
| Series statement | Dissertation ; |
| Volume/sequential designation | no. FB-24-01 |
| 500 ## - GENERAL NOTE | |
| General note | A dissertation submitted in partial fulfillment of the requirements for the degree of doctor of Philosophy in Food Engineering and Bioprocess Technology |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Asian Institute of Technology, 2024 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Microplastics increasingly infiltrate environmental systems and the food chain, severely impacting plastic pollution. Low-density polyethylene (LDPE), used in food containers, is a crucial challenge for environmental contamination. This study aimed to identify effective microorganisms for LDPE biodegradation, investigate the pathway, and develop strategies to enhance the efficiency of LDPE biodegradation. The primary screening, 22 bacteria and 4 fungi were isolated. Out of those, AS3 exhibited the highest LDPE percent weight reduction of 3.06 ± 0.52%, followed by strain AS8 showed 2.01 ± 0.20% after 4 weeks of incubation. The identification of AS3 showed 97.31% similarity to Bacillus toyonensis (MG561349.1), and AS8 exhibited 97.85% similarity to Sphingobacterium sp. (KJ411920.1). Bacillus sp. AS3 and Sphingobacterium sp. AS8 activities for LDPE degradation were quantitatively analysed. Bacillus sp. AS3 showed new functional groups formation on the LDPE film including N-O and C-O groups after incubation. The LDPE biodegradation pathway was projected from identifying depolymerized molecules, suggesting the presence of extracellular enzymes that break down LDPE into smaller molecules. Moreover, physicochemical pretreatment of LDPE before biodegradation demonstrated an effective strategy to enhance the LDPE biodegradation. The pretreated LDPE enhanced microbial biodegradability, improved biofilm formation, and significantly reduced the physical weight of LDPE. Bacillus sp. AS3 demonstrated an increasing LDPE weight loss of 5.06 ± 1.6%. However, the bacterial consortia AS3-8 strains (bacteria AS3 and AS8) illustrated the highest reduction weight of 8.01 ± 0.5% after 45 days of incubation. Consortia AS3-8 also exhibited the highest decrease in physical properties of LDPE significantly, with water contact angle (WCA) decreasing to 64.21 ± 3.69° and tensile strength (TS) decreasing to 17.97 ± 0.3 MPa. The esterase activity reached a maximum of 0.106 U/mL. SDS-PAGE analysis revealed proteins similar to known enzymes, such as laccase and esterase. This study addresses critical knowledge gaps in plastic biodegradation, focusing on the degradation of LDPE and enhancing biodegradation efficiency. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plastics |
| General subdivision | Biodegradation |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plastics |
| General subdivision | Environmental aspects |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microplastics |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Anal, Anil Kumar, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thammarat Koottatep, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Loc, Thai Nguyen, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Royal Thai Government Fellowship, |
| Relator term | Scholarship Donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | AIT Fellowship, |
| Relator term | Scholarship Donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Dissertation ; |
| Volume/sequential designation | no. FB-24-01 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B22779">http://203.159.5.9/ait-thesis/detail.php?q=B22779</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12464089 |
| b | mnarc |
| c | a |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 250919 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 250916 |
| First date, FD (RLIN) | m |
| -- | h |
| -- | a |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 67-Electronic Resource |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : - |
| CREATED | CREATED : 2025-11-09 |
| RECORD Id | RECORD # : i13557166 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 17/08/2026 | AIT Diss. no.FB-24-01 | 17/08/2026 | 1 | 17/08/2026 | 67-Electronic Resource |