Antioxidant activity and DNA damage protection by total carotenoids and vitamin C from fresh and cooked G. cowa leaves (Record no. 6283)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04554nas a2200397 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260817162802.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100604s2009 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12078347 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.FB-09-12 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rujira Chaiyasit |
| 245 10 - TITLE STATEMENT | |
| Title | Antioxidant activity and DNA damage protection by total carotenoids and vitamin C from fresh and cooked G. cowa leaves |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2009 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 77 leaves : |
| Other physical details | ill. + |
| Accompanying material | 1 online resource |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. no. FB-09-12 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Asian Institute of Technology, 2009 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The objective of this work was to study the anti-oxidant activity of G. cowa leaves, the changes that occurred during its processing and the protection it could provide to DNA from UV light and highly oxidative reagents like hydrogen peroxide. Initially the effect of extraction solvent on carotenoids content, ascorbic acid content and their antioxidant activity from fresh G. cowa leaves was studied. We extracted carotenoids from G. cowa fresh leaf, using a mixture of hexane, acetone and ethanol. Maximum amount of carotenoids content was obtained with mixture of hexane and ethanol (22.28 p-carotene/kg fresh weight). Antioxidant activity of the extracts obtained using this solvent was assessed by measuring DPPH radical scavenging (%), and found to 61.82%. Therefore, the mixture solvent of hexane and ethanol were select for further determination of total carotenoids content and antioxidant activity in fresh and cooked G. cowa leaves. Three types of processing of the leaves were done after cooking for 15 minutes at 100°C in boiling water, baking and steaming treatment, total carotenoids content and their antioxidant activity of boiling, baking and steaming was found to be different for each method. For boiling and baking, carotenoid content and their antioxidant activity increased initially and after being boiled for 5 minutes decreased. On steaming cooking, the carotenoid content and their antioxidant activity showed a continuously increased during steam from 3 minutes to 10 minutes and then decrease till the end of steaming time studied (15 minutes). Ascorbic acid extraction was best with 0.5% metaphospharic acid (155.41 mg ascorbic acidlkg fresh weight) and the percentage of DPPH radical was 85.81 %. The 0.5% metaphospharic acid use to determined content of G. cowa leaves after they were processed by boiling, baking and steaming at 100°C for 10 minutes. All methods had an effect on ascorbic acid content. Ascorbic acid content from boiling method decreased to 0.75 mg ascorbic acid/kg fresh weight, from baking method decreased to 1.81 mg ascorbic acidlkg fresh weight and for steaming method decreased to 64.35 mg ascorbic acidlkg fresh weight. A good relationship was observed between ascorbic acid content and antioxidant activity. In the study of DNA damage protection by carotenoids and ascorbic acid from fresh and cooked G. cowa leaves it was found that carotenoid extracts of G. cowa leaves after boiling, baking and steaming method could protect DNA from damage by hydrogen peroxide oxidation. However, ascorbic extracts of G. cowa leaves after boiling, baking and steaming method could not protect DNA from damage |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Antioxidants |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Vitamin C |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA damage |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rakshit, Sudip Kumar, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Athapol Noomhorm, |
| Relator term | Examination Committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yakupitiyage, Amararatne, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | RTG Fellowship, |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. no. FB-09-12 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B02740">http://203.159.5.9/ait-thesis/detail.php?q=B02740</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12078347 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240329 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 100604 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 20-AIT Publication |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120670210 |
| CREATED | CREATED : 2009-05-01 |
| RECORD Id | RECORD # : i12637324 |
| LPATRON | LPATRON : 1024122 |
| LCHKIN | LCHKIN : 2013-10-09 |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 1 |
| -- | TOT RENEW : 0 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120670202 |
| CREATED | CREATED : 2009-05-01 |
| RECORD Id | RECORD # : i12637336 |
| LPATRON | LPATRON : 1025967 |
| LCHKIN | LCHKIN : 2015-06-13 |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 2 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 17/08/2026 | 50.00 | AIT Thesis no.FB-09-12 | 30050120670210 | 17/08/2026 | 1 | 17/08/2026 | 20-AIT Publication | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 17/08/2026 | 50.00 | AIT Thesis no.FB-09-12 | 30050120670202 | 17/08/2026 | 2 | 17/08/2026 | 20-AIT Publication |

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