Antioxidant activity and DNA damage protection by total carotenoids and vitamin C from fresh and cooked G. cowa leaves (Record no. 6283)

MARC details
000 -LEADER
fixed length control field 04554nas a2200397 a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260817162802.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 100604s2009 th uu|m rtt 0| a1eng d
035 ## - SYSTEM CONTROL NUMBER
System control number .b12078347
099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number AIT Thesis no.FB-09-12
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Rujira Chaiyasit
245 10 - TITLE STATEMENT
Title Antioxidant activity and DNA damage protection by total carotenoids and vitamin C from fresh and cooked G. cowa leaves
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Pathum Thani, Thailand :
Name of publisher, distributor, etc. Asian Institute of Technology,
Date of publication, distribution, etc. 2009
300 ## - PHYSICAL DESCRIPTION
Extent 77 leaves :
Other physical details ill. +
Accompanying material 1 online resource
490 1# - SERIES STATEMENT
Series statement Thesis ;
Volume/sequential designation no. no. FB-09-12
500 ## - GENERAL NOTE
General note A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Asian Institute of Technology, 2009
520 ## - SUMMARY, ETC.
Summary, etc. The objective of this work was to study the anti-oxidant activity of G. cowa leaves, the changes that occurred during its processing and the protection it could provide to DNA from UV light and highly oxidative reagents like hydrogen peroxide. Initially the effect of extraction solvent on carotenoids content, ascorbic acid content and their antioxidant activity from fresh G. cowa leaves was studied. We extracted carotenoids from G. cowa fresh leaf, using a mixture of hexane, acetone and ethanol. Maximum amount of carotenoids content was obtained with mixture of hexane and ethanol (22.28 p-carotene/kg fresh weight). Antioxidant activity of the extracts obtained using this solvent was assessed by measuring DPPH radical scavenging (%), and found to 61.82%. Therefore, the mixture solvent of hexane and ethanol were select for further determination of total carotenoids content and antioxidant activity in fresh and cooked G. cowa leaves. Three types of processing of the leaves were done after cooking for 15 minutes at 100°C in boiling water, baking and steaming treatment, total carotenoids content and their antioxidant activity of boiling, baking and steaming was found to be different for each method. For boiling and baking, carotenoid content and their antioxidant activity increased initially and after being boiled for 5 minutes decreased. On steaming cooking, the carotenoid content and their antioxidant activity showed a continuously increased during steam from 3 minutes to 10 minutes and then decrease till the end of steaming time studied (15 minutes). Ascorbic acid extraction was best with 0.5% metaphospharic acid (155.41 mg ascorbic acidlkg fresh weight) and the percentage of DPPH radical was 85.81 %. The 0.5% metaphospharic acid use to determined content of G. cowa leaves after they were processed by boiling, baking and steaming at 100°C for 10 minutes. All methods had an effect on ascorbic acid content. Ascorbic acid content from boiling method decreased to 0.75 mg ascorbic acid/kg fresh weight, from baking method decreased to 1.81 mg ascorbic acidlkg fresh weight and for steaming method decreased to 64.35 mg ascorbic acidlkg fresh weight. A good relationship was observed between ascorbic acid content and antioxidant activity. In the study of DNA damage protection by carotenoids and ascorbic acid from fresh and cooked G. cowa leaves it was found that carotenoid extracts of G. cowa leaves after boiling, baking and steaming method could protect DNA from damage by hydrogen peroxide oxidation. However, ascorbic extracts of G. cowa leaves after boiling, baking and steaming method could not protect DNA from damage
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Antioxidants
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Vitamin C
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA damage
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Rakshit, Sudip Kumar,
Relator term Chairperson
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Athapol Noomhorm,
Relator term Examination Committee
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Yakupitiyage, Amararatne,
Relator term Examination Committee
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element RTG Fellowship,
Relator term Scholarship donor
810 2# - SERIES ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Asian Institute of Technology.
Title of a work Thesis ;
Volume/sequential designation no. no. FB-09-12
856 ## - ELECTRONIC LOCATION AND ACCESS
Materials specified Full-Text
Uniform Resource Identifier <a href="http://203.159.5.9/ait-thesis/detail.php?q=B02740">http://203.159.5.9/ait-thesis/detail.php?q=B02740</a>
907 ## - LOCAL DATA ELEMENT G, LDG (RLIN)
a .b12078347
b mnait
c u
902 ## - LOCAL DATA ELEMENT B, LDB (RLIN)
a 240329
998 ## - LOCAL CONTROL INFORMATION (RLIN)
Operator's initials, OID (RLIN) 0
Cataloger's initials, CIN (RLIN) 100604
First date, FD (RLIN) m
-- a
-- u
-- 0
945 ## - LOCAL PROCESSING INFORMATION (OCLC)
l mnait
945 ## - LOCAL PROCESSING INFORMATION (OCLC)
l mnait
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type 20-AIT Publication
909 ## - LOCAL ITEMS USED
Barcode Barcode : 30050120670210
CREATED CREATED : 2009-05-01
RECORD Id RECORD # : i12637324
LPATRON LPATRON : 1024122
LCHKIN LCHKIN : 2013-10-09
RENEWALS # RENEWALS : 0
-- # OVERDUE : 0
-- IUSE3 : 0
-- TOT CHKOUT : 1
-- TOT RENEW : 0
909 ## - LOCAL ITEMS USED
Barcode Barcode : 30050120670202
CREATED CREATED : 2009-05-01
RECORD Id RECORD # : i12637336
LPATRON LPATRON : 1025967
LCHKIN LCHKIN : 2015-06-13
RENEWALS # RENEWALS : 0
-- # OVERDUE : 0
-- IUSE3 : 0
-- TOT CHKOUT : 2
-- TOT RENEW : 0
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Cost, normal purchase price Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
      Available for Loans Asian Institute of Technology Library Asian Institute of Technology Library AIT Publications 17/08/2026 50.00   AIT Thesis no.FB-09-12 30050120670210 17/08/2026 1 17/08/2026 20-AIT Publication
      Available for Loans Asian Institute of Technology Library Asian Institute of Technology Library AIT Publications 17/08/2026 50.00   AIT Thesis no.FB-09-12 30050120670202 17/08/2026 2 17/08/2026 20-AIT Publication
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