AI SearchApplication of dielectrophoretic field-flow fractionation in environmental studies (Record no. 6546)
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|---|---|
| fixed length control field | 05536nas a2200433 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260817162848.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100531s2009 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12077434 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Diss. no.EV-08-14 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sittisak Pui-ock |
| 245 10 - TITLE STATEMENT | |
| Title | Application of dielectrophoretic field-flow fractionation in environmental studies |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2008 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 238 p. : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Dissertation ; |
| Volume/sequential designation | no. EV-08-14 |
| 500 ## - GENERAL NOTE | |
| General note | A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Technical Science in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2008 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Earlier studies suggest dielectrophoretic techniques may be applicable for rapid detection of toxicity by exploiting changes induced in the dielectric properties of cells as a result of exposure to toxic agents. However, existing dielectric methods, including electrorotation and dielectrophoretic crossover frequency measurements, are inconvenient, tedious and lengthy. The aim of this study was to develop a practical and speedy dielectrophoretic approach to in vitro toxicity testing. To achieve this, the current study developed several innovations in dielectrophoresis combined with field-flow fractionation (dFFF) using flow cytometry for cell detection. In dFFF, a micro-patterned, nano-film electrode imposes electric forces on cells suspended in a parabolic flow profile as they pass through a thin chamber. Relative changes in the dFFF peak elution times, expressed as a dielectric response index D;, reveal the responses that occur in cell membrane area (capacitance) and ion permeability (conductivity) caused by toxicant exposure. For our purposes, cells were exposed to eight toxicants, each of different single or mixed modes of action. Cell responses were studied over the first 150 minutes of exposure. The selected toxicants include acrylonitrile, actinomycin D, carbon tetrachloride, endosulfan, N-nitroso-N-methylurea (NMU), paraquat dichloride, puromycin, and styrene oxide. The human-derived leukemia cell line HL-60 was used as the in vitro mammalian cell toxicity detection model. For all chemical treatments except actinomycin D, the Di of the HL-60 cells increased in a dose- and time- dependent manner. The sensitivity of the dFFF method was compared with both the cell viability assay for all toxicants studied and either mitochondrial potentiometric dye assay or DNA alkaline comet assay, depending on each toxicant's specific mode of action. Except for low doses of nucleic acid-targeting agents (actinomycin D and NMU), the dFFF method detected all toxicants with more sensitivity than the other assays, often up to 10⁵ times more sensitive than the viability approach. Based on the octanollwater partition (Kow) characteristics of the chemicals tested, the lipophilic chemicals (those with high log Kow) proved to be more easily detected in terms of the dFFF response index Di; than the hydrophilic chemicals (those with low log Kow). The dFFF method appeared to be especially sensitive to agents that directly damaged the plasma membrane or induced apoptosis in the cells. Notably, dFFF detected significant changes in the cell plasma membrane even at the earliest stage of apoptosis induction. In addition, dFFF more acutely detected changes in the plasma membrane of cells exposed to toxicants at doses too low to induce apoptosis or loss of cell viability. Intriguingly, the cells were still stimulated, leading to an increased cell membrane area, perhaps in the form of additional microvilli. A dFFF sample preparation protocol was also developed for testing the toxicity of environmental water samples. Finally, the flow cytometer was replaced by a laser light scatter detector. This device allows the dFFF method to be realized in a more portable, accessible, and cost-effective configuration. Based upon all the findings of this study, it is concluded that the dFFF technique merits further study as a potential rapid in vitro toxicant screening tool |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Dielectrophoresis |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Human ecology |
| General subdivision | Study and teaching |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mathuros Ruchirawat, |
| Relator term | Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gascoyne, Peter R.C., |
| Relator term | Examination committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Preeda Pakpian, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jutamaad Satayavivad, |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Chulabhorn Research Institute, |
| Relator term | Scholarship donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Mahidol University, |
| Relator term | Scholarship donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology Fellowship, |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Dissertation ; |
| Volume/sequential designation | no. EV-08-14 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B00538">http://203.159.5.9/ait-thesis/detail.php?q=B00538</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12077434 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240329 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 100531 |
| First date, FD (RLIN) | m |
| -- | a |
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| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 22-AIT Thesis (Replacement) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120725691 |
| CREATED | CREATED : 2012-08-16 |
| RECORD Id | RECORD # : i12686724 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050160004239 |
| CREATED | CREATED : 2016-09-02 |
| RECORD Id | RECORD # : i12878959 |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 17/08/2026 | AIT Diss. no.EV-08-14 | 30050120725691 | 17/08/2026 | 3 | 17/08/2026 | 22-AIT Thesis (Replacement) | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 17/08/2026 | AIT Diss. no.EV-08-14 | 30050160004239 | 17/08/2026 | 17/08/2026 | 40-Archives |