AI SearchApplication of Trichoderma harzianum BCC5828 and N- acetylglucosaminidase enzyme as biocontrol agents (Record no. 6930)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03396nas|a2200409 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260817163003.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 060712s2005 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b11991598 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Thesis no.FB-05-21 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Than Than Sein |
| 245 10 - TITLE STATEMENT | |
| Title | Application of Trichoderma harzianum BCC5828 and N- acetylglucosaminidase enzyme as biocontrol agents |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2005 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 55 leaves : |
| Other physical details | ill. + |
| Accompanying material | 1 online resource |
| 490 1# - SERIES STATEMENT | |
| Series statement | Thesis ; |
| Volume/sequential designation | no. FB-05-21 |
| 500 ## - GENERAL NOTE | |
| General note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Eng.) - Asian Institute of Technology, 2005 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Trichoderina harziaiuun BCC5828, a mycoparasitic fungus and an enzyme of the chitinase enzyme complex, N- acetylglucosaminidase were tested as a biocontrol agent to control the development of three three pathogens, Fusarium oxysporum, Rizoctonia solani,Pythium aphaniderinatum. Initially Trichoderina strain was grown in liquid medium in shake flasks culture was induced to produce of N- acetylglucosaminidase enzyme in the presence of chitin. In a bioreactor the enzyme production was doubled with the same level of Chitin 1% as compared to shake flask cultures. The enzyme was purified by ammonium sulphate precipitation followed by CM cellulose chromatography, DEAE chromatography and Sephadex G-50 gel filtration. Overall a 32 fold increased in enzyme purity was obtained. In order to characterize the molecular weight of the N-acetylglucosaminidase enzyme was determined by SDS PAGE analysis and the was found to have a molecular weight of 35 KDa Maximal activities were determined at pH 6 and optimum temperature range of 50 °C to 60°C. The enzyme is stable at 25°C and 37°C and enhanced activity was obtained with iron salts. The K,,, and V max value were 4.15mM and 2.O4 mol/min of substrates. The enzyme was not found to be very effected as a biocontrol agent in well diffusion test done on three plant pathogens. However the fungi itself was found be very active and can be used as a good biocontrol against for the three plant pathogens studied |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Trichoderma |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Soilborne plant pathogens |
| General subdivision | Biological control |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rakshit, Sudip Kumar, |
| Relator term | Chairperson |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jindal, Vinod Kumar, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Athapol Noomhorm, |
| Relator term | Examination Committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Joint Japan I World Bank Graduate Scholarship Program, |
| Relator term | Scholarship Donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology Fellowship, |
| Relator term | Scholarship Donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Thesis ; |
| Volume/sequential designation | no. FB-05-21 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B12474">http://203.159.5.9/ait-thesis/detail.php?q=B12474</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b11991598 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 250326 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 060712 |
| First date, FD (RLIN) | m |
| -- | a |
| -- | u |
| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 22-AIT Thesis (Replacement) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120674170 |
| CREATED | CREATED : 2014-07-03 |
| RECORD Id | RECORD # : i12769332 |
| LPATRON | LPATRON : 1032873 |
| LCHKIN | LCHKIN : 2025-03-26 |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 3 |
| -- | TOT RENEW : 0 |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120229363 |
| CREATED | CREATED : 2016-04-19 |
| RECORD Id | RECORD # : i12937873 |
| LPATRON | LPATRON : 0 |
| LCHKIN | LCHKIN : - |
| RENEWALS | # RENEWALS : 0 |
| -- | # OVERDUE : 0 |
| -- | IUSE3 : 0 |
| -- | TOT CHKOUT : 0 |
| -- | TOT RENEW : 0 |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Cost, normal purchase price | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 17/08/2026 | 50.00 | AIT Thesis no.FB-05-21 | 30050120674170 | 17/08/2026 | 3 | 17/08/2026 | 22-AIT Thesis (Replacement) | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 17/08/2026 | AIT Thesis no.FB-05-21 | 30050120229363 | 17/08/2026 | 1 | 17/08/2026 | 40-Archives |