AI SearchPhysiological study of Ferric Uptake Regulator (FUR) gene in agrobacterium tumefaciens (Record no. 87894)
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| 000 -LEADER | |
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| fixed length control field | 04571nas a2200433 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260818171903.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 090602s2008 th uu|m rtt 0| a1eng d |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | .b12057381 |
| 099 #9 - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | AIT Diss. no.EV-08-10 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Worawan Kitphati |
| 245 10 - TITLE STATEMENT | |
| Title | Physiological study of Ferric Uptake Regulator (FUR) gene in agrobacterium tumefaciens |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Pathum Thani, Thailand : |
| Name of publisher, distributor, etc. | Asian Institute of Technology, |
| Date of publication, distribution, etc. | 2008 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 136 p. : |
| Other physical details | ill. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Dissertation ; |
| Volume/sequential designation | no. EV-08-10 |
| 500 ## - GENERAL NOTE | |
| General note | A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Inter-University Program on Environmen tal Toxicology, Technology and Management |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2008 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Iron is an essential metal for bacterial survival due to the fact that it is a required cofactor in a large number of iron-containing enzymes and proteins. However, when present in excess it can be toxic due to its ability to induce the generation of highly deleterious free radicals via the Fenton reaction. Therefore, intracellular iron concentrations need to be carefully adjusted by controlling iron transport, storage and consumption. In many bacteria, this regulation is mediated by the ferric uptake regulator (Fur) protein. Fur is a 17 KDa DNA binding protein which represses the transcription of many iron-responsive genes in Escherichia coli and other bacteria. Fur regulates not only iron-regulated genes but also genes involved in; metal homeostasis, the oxidative stress response and virulence. Agrobacterium tumefaciens is a soil-born plant pathogen that causes crown gall tumor in many dicotyledonous plants. The A. tumefaciens genome encodes 3 fur homologues; fur, irr and zur. In order to determine the functional role of A.tumefaciens Fur, an A. tumefaciens fur mutant was constructed by insertional inactivation. The mutant construction was confirmed by Southern blot analysis. The phenotypes of this mutant relating to metal homeostasis and the oxidative stress response were investigated. Atypically, A.tumefaciens Fur did show alterations in the levels of siderophore biosynthesis. However, the A. tumefaciens fur mutant was more sensitive to an iron chelator, 2,2-dipyridyl, and more resistant to an iron-activated antibiotic, streptonigrin, than the wild type A. tumefaciens NTL₄. This suggested that Fur has a role in regulating the intracellular iron concentration and that the A. tumefaciens fur mutant was iron-deficient. Apart from iron, A.tumefaciens Fur may also be involved in the regulation of divalent cation homeostasis. A. tumefaciens Fur was shown to playa role in the oxidative stress response. The A. tumefaciens fur mutant was hypersensitive to hydrogen peroxide and showed reduced catalase activity. Virulence assays showed that the A. tumefaciens fur mutant had a reduced ability to cause tumors on tobacco leaves compared to the wild type strain. The virulence deficiency of the A. tumefaciens fur mutant was proposed to be due, at least in part, to an impaired ability to cope with oxidative stress conditions caused primarily by a reduction in catalase levels |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Agrobacterium tumefaciens |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Skorn Mongkolsuk, |
| Relator term | Chairperson |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Preeda Pakpian, |
| Relator term | Examination Committee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Suvit Loprasert, |
| Relator term | Examination committee |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dubbs, James M., |
| Relator term | Examination committee |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Chulabhorn Research Institute, |
| Relator term | Scholarship donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Mahidol University, |
| Relator term | Scholarship donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology Fellowship, |
| Relator term | Scholarship donor |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Office of Higher Education Commission (OHEC), Thailand, |
| Relator term | Scholarship donor |
| 810 2# - SERIES ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Asian Institute of Technology. |
| Title of a work | Dissertation ; |
| Volume/sequential designation | no. EV-08-10 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Full-Text |
| Uniform Resource Identifier | <a href="http://203.159.5.9/ait-thesis/detail.php?q=B00534">http://203.159.5.9/ait-thesis/detail.php?q=B00534</a> |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) | |
| a | .b12057381 |
| b | mnait |
| c | u |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) | |
| a | 240329 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) | |
| Operator's initials, OID (RLIN) | 0 |
| Cataloger's initials, CIN (RLIN) | 090602 |
| First date, FD (RLIN) | m |
| -- | a |
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| -- | 0 |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnait |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) | |
| l | mnarc |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 22-AIT Thesis (Replacement) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | 40-Archives |
| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050120725709 |
| CREATED | CREATED : 2012-08-16 |
| RECORD Id | RECORD # : i12687261 |
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| 909 ## - LOCAL ITEMS USED | |
| Barcode | Barcode : 30050160004270 |
| CREATED | CREATED : 2016-09-02 |
| RECORD Id | RECORD # : i1287890x |
| LPATRON | LPATRON : 0 |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | AIT Publications | 18/08/2026 | AIT Diss. no.EV-08-10 | 30050120725709 | 18/08/2026 | 3 | 18/08/2026 | 22-AIT Thesis (Replacement) | ||||
| Available for Loans | Asian Institute of Technology Library | Asian Institute of Technology Library | Archives | 18/08/2026 | AIT Diss. no.EV-08-10 | 30050160004270 | 18/08/2026 | 18/08/2026 | 40-Archives |