Biostimulation activity of chitosan on the germination and growth rate of bean plant

By: Call Number: AIT Thesis no. BP-02-05 Contributor(s): Material type: SeriesSeries: Asian Institute of Technology. Thesis ; no. BP-02-05Publication details: Bangkok : Asian Institute of Technology, 2002Description: 63 leavesSubject(s): Online resources: Dissertation note: Thesis (M.Sc.) - Asian Institute of Technology, 2002 Summary: Biostimulation activity of chitosan on the germination rate of mung bean seeds has been investigated. The parameters studied for enhancing the germination rate in this work were different molecular weight, different temperature and different concentrations. The germination rate of seedling in each molecular weight were significant different to each other at seven days germination. The germination rate was found to be higher at 30°C. The germination rate of seedling does not depend on the concentrations of chitosan solution. Under the same condition and concentration, chitosan with molecular weight of 40 000 gave higher germination rate than those of 600 000; 1 000 000; acetic acid, D-glucosamine, N-acetylglucosamine and distilled water. The effect of three different concentrations of chitosan on the germination percentage of three kinds of bean seeds were investigated and found that germination percentage was higher at 20 ppm in yard long bean and soybean. The effect of spraying with four different concentrations of chitosan solution has been studied. It was found that growth rate, leaf area, number of nodules and number of flower buds were higher than that of untreated (control) plants. Similarly, effect of soaking seeds with chitosan solution has been studied. It was found that growth rate, leaf area, number of nodules formed, number o'f flower buds produced and dry weight of bean fruits were higher by using chitosan soaked seeds as compared to unsoaked seeds. When comparing the effect of treatments by soaking seeds before sowing and spraying on leaves, no significant effect was found on growth rate by applying 10, 15 and 20 ppm between two treatments (p>0.05). However, spraying with 1 ppm showed significant effect on growth rate and also on leaf area (p<0.05). Analysis on plant nutrients in mung bean leaves was also studied and found that magnesium content was significantly higher by spraying with 1 ppm chitosan solution.
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Thesis (M.Sc.) - Asian Institute of Technology, 2002

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development

Biostimulation activity of chitosan on the germination rate of mung bean seeds has been investigated. The parameters studied for enhancing the germination rate in this work were different molecular weight, different temperature and different concentrations. The germination rate of seedling in each molecular weight were significant different to each other at seven days germination. The germination rate was found to be higher at 30°C. The germination rate of seedling does not depend on the concentrations of chitosan solution. Under the same condition and concentration, chitosan with molecular weight of 40 000 gave higher germination rate than those of 600 000; 1 000 000; acetic acid, D-glucosamine, N-acetylglucosamine and distilled water. The effect of three different concentrations of chitosan on the germination percentage of three kinds of bean seeds were investigated and found that germination percentage was higher at 20 ppm in yard long bean and soybean. The effect of spraying with four different concentrations of chitosan solution has been studied. It was found that growth rate, leaf area, number of nodules and number of flower buds were higher than that of untreated (control) plants. Similarly, effect of soaking seeds with chitosan solution has been studied. It was found that growth rate, leaf area, number of nodules formed, number o'f flower buds produced and dry weight of bean fruits were higher by using chitosan soaked seeds as compared to unsoaked seeds. When comparing the effect of treatments by soaking seeds before sowing and spraying on leaves, no significant effect was found on growth rate by applying 10, 15 and 20 ppm between two treatments (p>0.05). However, spraying with 1 ppm showed significant effect on growth rate and also on leaf area (p<0.05). Analysis on plant nutrients in mung bean leaves was also studied and found that magnesium content was significantly higher by spraying with 1 ppm chitosan solution.

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