AI SearchAntioxidant activity and protective effect on DNA cleavage of some natural products
Call Number: AIT Thesis no.PH-03-8 Material type:
SeriesSeries: Asian Institute of Technology. Thesis ; no. PH-03-8Publication details: Pathum Thani, Thailand : Asian Institute of Technology, 2003Description: 45 leaves : illSubject(s): Online resources: Dissertation note: Thesis (M.Sc.) - Asian Institute of Technology, 2003 Summary: Comparison of antioxidant activity (AOA) of y-oryzonol present in dehusked rice and raw and stabilized rice bran by DPPH radical scavenging and hydroxyl radical scavenging method were done. The AOA of dehusked rice bran oil (DHRBO), raw rice bran oil (RRBO-extracted with hexane) and RRBO* (extracted with hexane : isopropanol (50:50)) and stabilized rice bran oil (SRBO) as measured by 1,1-Diphenyl-2-picryhydrazyl (DPPH) radical scavenging method were in the range of 37-46% at the concentration of 50 mg/ml. The AOA of different crude rice bran samples estimated by deoxyribose oxidation method also did not differ much (24-35%). These results indicated that processing of rice bran in different ways have little effect on AOA of y-oryzonol presents in rice bran oil. The leaves of Thai basil (Ocimum sanctum L) were freeze-dried and extracted with water, methanol, ethyl acetate and hexane. The anioxidant properties of leaf extracts and protective effect on DNA cleavage were tested. The antioxidant properties of basil leaf extracts were compared to the activityof ascorbic acid (AsA) and butylated hydroxyanisole (BHA). DPPH radical scavenging activities of the ethyl acetate fraction (EAFB) and hexane fraction of basil leaf (HFB) exhibited more DPPH radical scavenging activity than that of BHA and AsA at all concentration (0.02-10 mg/ml) tested. The water fraction (WFB) showed much higher DPPH radical scavenging activity than that of other antioxidants at low concentrations (0.02 - 1 mg/ml), while methanol fraction of basil showed little activity. The ethyl acetate and hexane fractions showed around 50 % inhibition of 2-deoxyribose oxidation by scavenging {u2022}OH at 10 mg/ml concentration, which was nearly same as compared to BHA (52.2 %) and AsA (53.46%). The water fraction showed 38.72 % antioxidant activity (AOA) at that concentration. Among the four fractions, water fraction of basil extract showed excellent protective effect on DNA cleavage followed by methanol fraction. The ethyl acetate fraction and hexane fraction had no protective effect. Water fraction of basil at 1.25 mg/ml concentration was found to be the minimum concentration that could prevent DNA strand scission.
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A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science
Thesis (M.Sc.) - Asian Institute of Technology, 2003
Comparison of antioxidant activity (AOA) of y-oryzonol present in dehusked rice and raw and stabilized rice bran by DPPH radical scavenging and hydroxyl radical scavenging method were done. The AOA of dehusked rice bran oil (DHRBO), raw rice bran oil (RRBO-extracted with hexane) and RRBO* (extracted with hexane : isopropanol (50:50)) and stabilized rice bran oil (SRBO) as measured by 1,1-Diphenyl-2-picryhydrazyl (DPPH) radical scavenging method were in the range of 37-46% at the concentration of 50 mg/ml. The AOA of different crude rice bran samples estimated by deoxyribose oxidation method also did not differ much (24-35%). These results indicated that processing of rice bran in different ways have little effect on AOA of y-oryzonol presents in rice bran oil. The leaves of Thai basil (Ocimum sanctum L) were freeze-dried and extracted with water, methanol, ethyl acetate and hexane. The anioxidant properties of leaf extracts and protective effect on DNA cleavage were tested. The antioxidant properties of basil leaf extracts were compared to the activityof ascorbic acid (AsA) and butylated hydroxyanisole (BHA). DPPH radical scavenging activities of the ethyl acetate fraction (EAFB) and hexane fraction of basil leaf (HFB) exhibited more DPPH radical scavenging activity than that of BHA and AsA at all concentration (0.02-10 mg/ml) tested. The water fraction (WFB) showed much higher DPPH radical scavenging activity than that of other antioxidants at low concentrations (0.02 - 1 mg/ml), while methanol fraction of basil showed little activity. The ethyl acetate and hexane fractions showed around 50 % inhibition of 2-deoxyribose oxidation by scavenging {u2022}OH at 10 mg/ml concentration, which was nearly same as compared to BHA (52.2 %) and AsA (53.46%). The water fraction showed 38.72 % antioxidant activity (AOA) at that concentration. Among the four fractions, water fraction of basil extract showed excellent protective effect on DNA cleavage followed by methanol fraction. The ethyl acetate fraction and hexane fraction had no protective effect. Water fraction of basil at 1.25 mg/ml concentration was found to be the minimum concentration that could prevent DNA strand scission.
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