03370nas|a2200277 i 450000500170000000800410001703500150005810000240007324500670009726000670016430000220023149000270025350200570028050001840033752021750052165000150269665000160271165000130272770000390274070000430277970000500282270000500287271000460292281000590296885600650302720260817171302.0040430s2003    th  u m rtt  00|  a1eng d  a.b119240560 aSiravit Charoenying10aBenefits and risks of y-irradiation for Curcuma longa products  aPathum Thani, Thailand :bAsian Institute of Technology,c2003  a66 leaves :bill.1 aThesis ;vno. EV-03-19  aThesis (M.Sc.) - Asian Institute of Technology, 2003  aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science. Inter-University Program on Environmental Toxicology, Technology and Management.  aIonizing irradiation is an alternative process for microbial decontamination for spices and herbs including Curcuma longa products. Gamma irradiation at the doses of I 0 and 25 kGy is usually practiced for herbs and pharmaceuticals, respectively. However, irradiation technique is suspected to alter the composition of some of the C. longa products. This study attempts to examine the formation of any new compounds after the gammairradiation process and also to optimize the irradiation of C. longa products at lower dose in order -to prevent any possible formation of new compounds which may cause the toxic effects. Five various sources of C. longa products (non-irradiated), including two samples from "one tambon-one product (OTOP)" project, were irradiated with Cobalt-60 gamma ray at the doses of 2.5, 5, I 0 and 25 kGy. Four different batches of commercial irradiated products (capsule) from one company were analyzed. Determination of heavy metals contaminated in C. longa products i.e. cadmium, arsenic, mercury, lead and iron were also performed. The pattern of Thin Layer Chromatography (TLC) with phosphomolybdic spraying showed no considerable changes of TLC patterns between non-irradiated (non-treatment) and irradiated (treatment) samples and without any new band. However, TLC pattern of the four commercial irradiated capsules showed that there was at least one TLC band different from other samples. This may be due to other excipients or diluents added in the capsule during manufacturing process. Irradiation at the dose of I 0 and 25 kGy completely eliminated the microorganisms and the dose of 5 kGy reduced the total viable number of bacteria and fungi down to limited values, whereas 2.5 kGy was less effective. According to these results, it is indicated that gamma-irradiation at the dose range of 5-10 kGy depending on bioberden is sufficient to decontaminate the microorganisms in C. longa products without any changes on the active compounds. High levels of iron (Fe) and cadmium (Cd) were also found in some C. longa products. Further study should be conducted to evaluate the toxicity of crude or extract of irradiated C. longa products. 0aGamma rays 0aIrradiation 0aTurmeric0 aJutamaad Satayavivad,eChairperson0 aPreeda Pakpian,eExamination Committee0 aPaiboon Vattanaviboon,eExamination committee0 aNuchanart Rangkadilok,eExamination committee2 aHer Majesty the Queen,eScholarship donor2 aAsian Institute of Technology.tThesis ;vno. EV-03-19  3Full-Textuhttp://203.159.5.9/ait-thesis/detail.php?q=B08242