04054cas|a2200421 i 4500 20260819085107.0 230399s1993 th uzm rtt 00| a1eng d .b10740831 AIT Thesis no. EV-93-28 Misra, Girish Chandra Transport and fate of benzene in a saturated groundwater environment - laboratory column study Bangkok : Asian Institute of Technology, 1993 78 leaves + 1 online resource Thesis ; no. EV-93-28 A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering Thesis (M.Eng.) - Asian Institute of Technology, 1993 Petroleum hydrocarbons from leaking underground storage tanks, accidental spillage or leachate from waste-dump sites have tremendous potential to contaminate the underground water bodies which serve as the primary source of drinking water for the majority of human population around the world. These chemicals, while traversing through the vadose zone, undergo physical, chemical and biological changes with significant depletion of concentration before finally reaching the aquifer. Microbial activity occurs to a varying degree within the aquifer too, although in most cases it is limited by either substrate deficiency, low nutrient availability or lack of electron acceptors. In this study mixed aerobic culture obtained from an activated sludge plant and later enriched with benzene degrading bacteria, was tested under batch condition (in serum bottle) and continuous flow soil columns for determining the extent of benzene biodegradation. Pure benzene as well as benzene present in gasoline were tested as carbon source. Results indicated a zero order rate of 0.9 mg/l.d for pure benzene and about 0.76 mg/l.d for gasoline. The effect of cosubstrate (glucose) on benzene biodegradation and toxicity of benzene on microbes were also studied. Presence of cosubstrate did not effect benzene degradation rate significantly. At concentration ranges tested (32.3-167.18 mg/I) benzene completely inhibited biomass growth. Results of column test indicated that benzene can be degraded in groundwater environment provided sufficient time is given for microbial acclimation and the presence of dissolved oxygen is ensured. Transport parameters estimated included the coefficient of dispersion and adsorption. Adsorption is found to be an important phenomenon which can significantly retard movement of benzene contaminated plume. The presence of fine soil particles such as clay and silt is pivotal in determining the extent of adsorption occurring in subsurface. The study provided valuable information for use in groundwater models to predict vertical penetration of aqueous phase benzene in aquifers. Water, Underground Microbiology Benzene Biodegradation Chongrak Polprasert, Chairperson Gupta, Ashim Das, Co-Chairperson Lee, Seong-Key, Examination committee Royal Thai Government (King's Scholarship), Scholarship donor Asian Institute of Technology. Thesis ; no. EV-93-28 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B16678 .b10740831 mnait x 240402 2 951012 m a x 0 mnait mnait mnarc 20 40 Barcode : 30050004965934 CREATED : 1994-05-19 RECORD # : i10950291 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : 30050003047429 CREATED : 1994-05-19 RECORD # : i10950308 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : 30050160055702 CREATED : 2016-11-05 RECORD # : i12975205 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 122618 122618 0 0 0 0 MAIN MAIN mnait 2026-08-19 0 AIT Thesis no. EV-93-28 30050004965934 2026-08-19 08:51:07 1 2026-08-19 20 0 0 0 0 MAIN MAIN mnait 2026-08-19 0 AIT Thesis no. EV-93-28 30050003047429 2026-08-19 08:51:07 2 2026-08-19 20 0 0 0 0 MAIN MAIN mnarc 2026-08-19 0 AIT Thesis no. EV-93-28 30050160055702 2026-08-19 08:51:07 2026-08-19 40