01906cas a2200253   450000500170000000800410001703500150005810000190007324500850009226000520017730000140022949000270024350001520027050200570042252008160047965000170129565000210131270000400133370000530137370000450142671000570147181000590152885600650158720260818084325.0980316s1997    th uu m rtt   000 a eng d  a.b116994621 aSheng, Haiqing10aComparison of the genetic structure of four tilapia strains using mtDNA analyses  aBangkok :bAsian Institute of Technology,c1997  a52 leaves1 aThesis ;vno. AS-97-14  aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development  aThesis (M.Sc.) - Asian Institute of Technology, 1997  aMitochondrial DNA (mtDNA) was isolated from four commercial strains of tilapia (Orechromis niloticus): AIT, CHITRALADA, GIFT and UDORN strain hold in the Agricultural and Aquatic System Program (AASP) at AIT. Restriction endonuclease analysis of mtDNA has been carried out to compare genetic structure and characterize differentiation in the four strains. The molecular size of mtDNA of these strains was close to 14500 base pairs. The mtDNA was digested by six hexanucleotide restriction enzymes and analyzed by agarose gel electrophoresis with ethidium bromide staining to reveal the restriction patterns of mtDNA. The results show that the fragment patterns produced by two enzymes (Apa I and Dra I) could be used as genetic markers for identification purposes among the four strains of Orechromis niloticus. 0aNile tilapia 0aFishesxGenetics1 aStevens, Willem Frans,eChairperson1 aYakupitiyage, Amararatne,eExamination Committee1 aLittle, David C.,eExamination Committee2 aThe Royal Netherlands Government,eScholarship donor2 aAsian Institute of Technology.tThesis ;vno. AS-97-14  3Full-Textuhttp://203.159.5.9/ait-thesis/detail.php?q=B13507