04474cas|a2200433 a 4500 20260818094856.0 020125s2001 th uu|m rtt 0| a1eng d .b11842842 AIT Thesis no.EV-01-7 Yoga, Gunawan Pratama Effect of cadmium exposure on the metallothionein induction and lipid peroxidation in Tilapia fish Bangkok : Asian Institute of Technology, 2001 66 leaves Thesis ; no. EV-01-7 A thesis submitted in partial fulfillment of the requirements for degree of Master of Science. Inter-university Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development Thesis (M.Sc.) - Asian Institute of Technology, 2001 The effect of cadmium exposure on the metallothionein (MT) induction in Tilapia fish has been studied. The aim of this study was to evaluate the effect of cadmium exposure on the MT induction and lipid peroxidation production in Tilapia in an attempt to evaluate the potential usefulness of the two parameters as biomarker of exposure and biomarker of effect, respectively. The preliminary study was performed to find the appropriate range of cadmium concentrations for exposure to the fish. The research was divided into two studies, dose-response relationship and time-course study, which were conducted simultaneously. Then the effects of cadmium exposure on metallothionein induction and on lipid peroxidation were conducted in the short-te1m and long-term period. Experimentally, fish were exposed to various levels of cadmium over a short-term period of up to seven days or over a long-term period of up to fom weeks. The levels of cadmium accumulated, metallothionein expression, malondialydehyde production which indicates lipid peroxidation were monitored. Cadmium accumulation in fish liver was concentration and time dependent during the short-term and long-term period as well, particularly at high level of concentrations, between 0.50-ppm, and 1.00-ppm. MT mRNA expression appeared at the seventh day after exposure and showed concentration dependence to the cadmium concentration in water. During the long-term period MT mRNA expression in fish liver were concentration dependent, but not time dependent. The MT mRNA expression was linearly co1Telated to cadmium accumulation in fish liver during the short-term period, but not in the long-te1m. In the short-term period, MDA production was dependent on cadmium accumulation in fish liver, but not in long-term period. In the short-term period, MDA production increased proportionally with time and concentration of cadmium. The highest MDA level was reached in the 1.00-ppm concentration level at the seventh day. But during the long-term study the MDA productions varied. Cadmium accumulation in fish liver correlated with MDA production in this organ during the short-term period. But during the longterm period the coITelation decreased. A positive coITelation was observed between MT mRNA expression and MDA production in the liver during the short-term period, but not in the longterm study. Tilapia Fishes Effect of heavy metals on Cadmium Environmental aspects Mathuros Ruchirawat, Chairperson Panida Nawasumrit, Examination committee Preeda Pakpian, Examination Committee Government of Germany (DAAD), IeScholarship donor Asian Institute of Technology. Thesis; no. EV-01-7 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B08202 .b11842842 mnait u 240329 0 020125 m a u 0 mnait mnait mnarc 20 40 Barcode : 30050120652192 CREATED : 2000-08-28 RECORD # : i1226779x LPATRON : 1017059 LCHKIN : 2005-08-22 # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 4 TOT RENEW : 4 Barcode : 30050120652184 CREATED : 2000-08-28 RECORD # : i12267806 LPATRON : 1012595 LCHKIN : 2002-04-19 # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 2 TOT RENEW : 1 Barcode : 30050160077102 CREATED : 2016-05-24 RECORD # : i12989447 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 34172 34172 0 0 0 0 MAIN MAIN mnait 2026-08-18 50.00 0 AIT Thesis no.EV-01-7 30050120652192 2026-08-18 09:48:56 1 2026-08-18 20 0 0 0 0 MAIN MAIN mnait 2026-08-18 50.00 0 AIT Thesis no.EV-01-7 30050120652184 2026-08-18 09:48:56 2 2026-08-18 20 0 0 0 0 MAIN MAIN mnarc 2026-08-18 0 AIT Thesis no.EV-01-7 30050160077102 2026-08-18 09:48:56 2026-08-18 40