04683nas a2200457 a 4500 20260818095308.0 110606s2010 th uu|m rtt 0| a1eng d .b12096404 AIT Diss. no.EV-10-06 Tanvisith Charoenying Effects of pesticides on the non-neuronal lymphocytic cholinergic system Pathum Thani, Thailand : Asian Institute of Technology, 2010 118 p. : ill. Dissertation ; no. EV-10-06 A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidal University, 2010 Concerns over adverse effects of pesticide -induced immunotoxic effects are heightened; however , knowledge on this area is relatively still limited . Subsequent to the discovery of non- neuronal cholinergic system in lymphocytes; suggesting that it might be susceptible to pesticides exposure. Pesticides namely, permethrin (PM), deltamethrin (DM), pa raquat (PQ), and paraoxon (PO) were selected to study for their immuno toxic effects. PM and PQ were studied in mice challenged with Plasmodium berghei . The resul ts showed that survival time of mice was significantly reduced and parasitemia percentages of PM- treated groups were slightly higher than control group as a result of prior oral exposure to PM for 14 days and during malaria infection . In contrast, PQ did not increase host susceptibility to Plasmodium infection in PQ -treated mice. The in vitro study showed that all selected pesticides reduced viability of SH -SY5Y, human neuroblastoma and MOLT -3, human leukemic T -lymphocytes cells. The effects of PO were fur ther investigated in SH -SY5Y and MOLT -3 cells. PO were able to induce change s of cholinergic system in both cells in a more or less similar response profile demonstrated by over -expression of AChE, a marker of apoptosis, alterations of M1, M2, and M3 mAChR s mRNA or protein s, and activation of the 2early responsive gene3 c -Fos gene expression. Moreover, the PO -induced activation N -AChE and c -Fos in both neuronal and non- neuronal cells were able to p artially be blocked by atropine indicating that mAChRs activ ation is partially involved in the PO -mediated cellular responses. Taken together, the results suggest that certain neurotoxic pesticides can elicit neurotoxic and immunotoxic effects. The similarity of the response profile in both neuronal and non- neuronal cells suggest that biological functions of such components are, more or less, alike. This present study provides initial and additional information in whic h non- neuronal lymphocytic muscarinic receptors are possible potential targets for pesticides -indu ced immunotoxic effects. In addition the present study also strengthens the belief of using lymphocytes as a surrogate marker for evaluating neurotoxic condition as the consequence of certain pesticides exposure. Pesticides Physiological effect Mice Effect of pesticides on Jutamaad Satayavivad, Chairperson Preeda Pakpian, Examination Committee Apinya Thiantanawat, Examination committee Sansanee Chaiyaroj, Examination committee Chulabhorn Research Institute, Scholarship donor Mahidol University, Scholarship donor Asian Institute of Technology Fellowship, Scholarship donor Royal Thai Government, Scholarship donor Asian Institute of Technology. Dissertation ; no. EV-10-06 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B00562 .b12096404 mnait u 240403 0 110606 m a u 0 000:001:PDF:b1209640:000659:0:0:0:0:0:0 Abstract-AIT Diss. no.EV-10-06 n mnait mnarc 22 40 Barcode : 30050120553788 CREATED : 2012-08-16 RECORD # : i12687443 LPATRON : 1021581 LCHKIN : 2013-12-07 # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 1 TOT RENEW : 0 Barcode : 30050120405799 CREATED : 2017-04-20 RECORD # : i13074738 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 35276 35276 0 0 0 0 MAIN MAIN mnait 2026-08-18 0 AIT Diss. no.EV-10-06 30050120553788 2026-08-18 09:53:08 3 2026-08-18 22 0 0 0 0 MAIN MAIN mnarc 2026-08-18 50.00 0 AIT Diss. no.EV-10-06 30050120405799 2026-08-18 09:53:08 2026-08-18 40