Sakpal, Prathama Vijay creator Salin, Krishna R. Chairperson Ha, Thanh Dong Examination Committee Yaseen, Muhammad Examination Committee AIT Scholarship Scholarhip Donor text series theses th Pathum Thani, Thailand Asian Institute of Technology 2025 continuing eng

print

45 leaves : ill.+ 1 online resource This study investigated the effects of dietary Withania somnifera (ashwagandha) root powder on the growth performance, haematological responses, antioxidant capacity, and stress physiology of butter catfish (Ompok pabda) reared in a Recirculating Aquaculture System (RAS). Four diets containing 0%, 1%, 2%, and 3% ashwagandha were tested at two stocking densities, 40 and 60 fish per 50L tank, corresponding to 0.8 and 1.2 fish/L, respectively over a 45-day feeding trial. Growth performance was primarily influenced by stocking density, with fish at 40 (lower density) exhibiting higher weight gain, better feed conversion ratio, and improved specific growth rate compared to those at 60 (higher density), while ashwagandha supplementation did not produce significant changes in growth parameters. Haematological indices (Hb, RBC, WBC) remained stable across all diet and density treatments, indicating that ashwagandha up to 3% inclusion did not induce hematological stress or immune suppression. Total antioxidant capacity (T-AOC) increased significantly in fish fed 2% and 3% ashwagandha, demonstrating enhanced endogenous antioxidant defence, whereas stocking density did not affect T-AOC. Serum glucose levels were higher in fish reared at the higher density, reflecting density-related stress; however, dietary ashwagandha did not significantly alter glucose concentrations across treatments during the 45 days. Overall, the results indicate that while ashwagandha did not markedly improve growth or haematological profiles under non-stressful conditions, it enhanced antioxidant capacity and maintained physiological safety, suggesting potential benefits under stress-inducing environments or with longer feeding durations. These findings highlight ashwagandha{u2019}s relevance as a natural, eco-friendly feed additive supporting health and resilience in O. pabda aquaculture, contributing to sustainable intensification and reduced reliance on synthetic inputs. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management Thesis (M. Sc.) - Asian Institute of Technology, 2025 Aquatic ecology Catfishes Asian Institute of Technology. http://203.159.5.9/ait-thesis/detail.php?q=B24087 http://203.159.5.9/ait-thesis/detail.php?q=B24087 260513 20260818095327.0