Evaluation of immobilization protocols of a fluorescence-based immunosensor used to detect infectious diseases
- Pathum Thani : Asian Institute of Technology, 2022
- 58 p. : ill.
- Thesis ; no. ISE-22-13 .
- Asian Institute of Technology. Thesis ; no. ISE-22-13 .
A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Nanotechnology
Thesis (M. Eng.) - Asian Institute of Technology, 2022
Infectious diseases are one of the main problems that cause rapid morbidity or mortality, especially with emerging epidemics. The rapid detection of infectious diseases is an essential factor in preventing, diagnosing, and treating transmission. Therefore, the design and fabrication of biosensors for detecting pathogens is one of the critical factors for promptly screening and identifying infected people. Immunosensors are a class of biosensors relying on antibody-antigen interactions, which have an attractive option for developing diagnostic tools such as home use and Point-of-Care (POC). Metal-organic frameworks (MOFs), thanks to their large surface area, high porosity, structural diversity, and tunable composition, metal-organic frameworks (MOFs) can help improve antibody immobilization efficiency to increase stability and controllable orientation. In particular, a subset represented by zeolitic imidazolate framework-C (ZIF-C), consisting of Zn2+ and 2-methylimidazole, can be produced around the Fc region of the antibody since Zn2+ accumulates on the negatively Fc region. Therefore, the Fc regions are partially embedded within the MOF, and the antibody-binding areas protrude toward the exterior part of the material. In this study, a model immunosensor based on an anti-albumin antibody will be optimized by using selective growth of oriented Abs into the ZIF-C nanocrystals on ZnO nanorods substrate (ZnO@ZIF-C*Ab). The efficiency, surface structures, and chemical elements of the immunosensor will be evaluated by Scanning Electron Microscopy (SEM), Energy Dispersion X-ray Spectroscopy (EDX), UV-VIS absorption spectroscopy, and Fluorescence spectroscopy on the FITC-tagged albumin.