03467nas a2200397 a 4500 20260818112739.0 230203s2022 th a ||| 000 eng d .b12398305 AIT Thesis no.ISE-22-13 Wisanu Simalai Evaluation of immobilization protocols of a fluorescence-based immunosensor used to detect infectious diseases Pathum Thani : Asian Institute of Technology, 2022 58 p. : ill. Thesis ; no. ISE-22-13 Thesis (M. Eng.) - Asian Institute of Technology, 2022 A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Nanotechnology Infectious diseases are one of the main problems that cause rapid morbidity or mortality, especially with emerging epidemics. The rapid detection of infectious diseases is an essential factor in preventing, diagnosing, and treating transmission. Therefore, the design and fabrication of biosensors for detecting pathogens is one of the critical factors for promptly screening and identifying infected people. Immunosensors are a class of biosensors relying on antibody-antigen interactions, which have an attractive option for developing diagnostic tools such as home use and Point-of-Care (POC). Metal-organic frameworks (MOFs), thanks to their large surface area, high porosity, structural diversity, and tunable composition, metal-organic frameworks (MOFs) can help improve antibody immobilization efficiency to increase stability and controllable orientation. In particular, a subset represented by zeolitic imidazolate framework-C (ZIF-C), consisting of Zn2+ and 2-methylimidazole, can be produced around the Fc region of the antibody since Zn2+ accumulates on the negatively Fc region. Therefore, the Fc regions are partially embedded within the MOF, and the antibody-binding areas protrude toward the exterior part of the material. In this study, a model immunosensor based on an anti-albumin antibody will be optimized by using selective growth of oriented Abs into the ZIF-C nanocrystals on ZnO nanorods substrate (ZnO@ZIF-C*Ab). The efficiency, surface structures, and chemical elements of the immunosensor will be evaluated by Scanning Electron Microscopy (SEM), Energy Dispersion X-ray Spectroscopy (EDX), UV-VIS absorption spectroscopy, and Fluorescence spectroscopy on the FITC-tagged albumin. Infectious diseases Biosensors Materials Ricco, Raffaele, Chairperson Bora, Tanujjal, Examination committee Trau, Dieter Wilhelm, Examination committee Royal Thai Government Fellowships, Scholarship Donor Asian Institute of Technology. Thesis ; no. ISE-22-13 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B18188 .b12398305 mnait m 250421 0 230203 m a m 0 mnarc mnarc 67 40 Barcode : - CREATED : 2023-01-02 RECORD # : i13429036 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : 30050121041650 CREATED : 2023-02-13 RECORD # : i13432126 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 41028 41028 0 0 0 0 MAIN MAIN mnarc 2026-08-18 0 AIT Thesis no.ISE-22-13 2026-08-18 11:27:39 2026-08-18 67 0 0 0 0 MAIN MAIN mnarc 2026-08-18 0 AIT Thesis no.ISE-22-13 30050121041650 2026-08-18 11:27:39 1 2026-08-18 40