TY  - SER
AU  - Wisanu Simalai
AU  - Ricco,Raffaele
AU  - Bora,Tanujjal
AU  - Trau,Dieter Wilhelm
ED  - Royal Thai Government Fellowships,
TI  - Evaluation of immobilization protocols of a fluorescence-based immunosensor used to detect infectious diseases
T2  - Thesis
PY  - 2022///
CY  - Pathum Thani
PB  - Asian Institute of Technology
KW  - Infectious diseases
KW  - Biosensors
KW  - Materials
N1  - A thesis submitted in partial fulfillment of the requirements for the degree of  Master of Engineering in Nanotechnology; Thesis (M. Eng.) - Asian Institute of Technology, 2022
N2  - Infectious diseases are one of the main problems that cause rapid morbidity or  mortality, especially with emerging epidemics. The rapid detection of infectious  diseases is an essential factor in preventing, diagnosing, and treating transmission.  Therefore, the design and fabrication of biosensors for detecting pathogens is one of  the critical factors for promptly screening and identifying infected people.  Immunosensors are a class of biosensors relying on antibody-antigen interactions,  which have an attractive option for developing diagnostic tools such as home use and  Point-of-Care (POC). Metal-organic frameworks (MOFs), thanks to their large surface  area, high porosity, structural diversity, and tunable composition, metal-organic  frameworks (MOFs) can help improve antibody immobilization efficiency to increase  stability and controllable orientation. In particular, a subset represented by zeolitic  imidazolate framework-C (ZIF-C), consisting of Zn2+ and 2-methylimidazole, can be  produced around the Fc region of the antibody since Zn2+ accumulates on the negatively  Fc region. Therefore, the Fc regions are partially embedded within the MOF, and the  antibody-binding areas protrude toward the exterior part of the material. In this study,  a model immunosensor based on an anti-albumin antibody will be optimized by using  selective growth of oriented Abs into the ZIF-C nanocrystals on ZnO nanorods  substrate (ZnO@ZIF-C*Ab). The efficiency, surface structures, and chemical elements  of the immunosensor will be evaluated by Scanning Electron Microscopy (SEM),  Energy Dispersion X-ray Spectroscopy (EDX), UV-VIS absorption spectroscopy, and  Fluorescence spectroscopy on the FITC-tagged albumin
UR  - http://203.159.5.9/ait-thesis/detail.php?q=B18188
ER  -