02720nas a2200277 a 450000500170000000800410001703500150005810000200007324500970009326000670019030000430025749000260030050002130032650200580053952014210059765000250201865000170204365000220206070000350208270000450211770000460216270000420220871000640225081000620231485600660237620260818113222.0190322s2015    th uu m rtt   0|  a1eng d  a.b1229990x1 aDhakal, Damodar10aExtraction and characterization of collagen to develop biopolymeric fiber from chicken feet.  aPathum Thani, Thailand :bAsian Institute of Technology,c2015  a83 leaves :bill. +e1 online resource1 aThesis ;vno.FB-15-09  aA thesis submitted  in  partial  fulfillment  of the  requirements  for  the degree  of Master  of Science  in  Food  Engineering  and  Bioprocess  Technology, School of Environment, Resources and Development  aThesis (M. Sc.) - Asian Institute of Technology, 2015  a Collagen was extracted sequencially from the chicken feet using papain, acetic acid and NaCl solution.  The optimum condition was found to be 30 ⁰ C temperature treatment and ( 25 ml/mg )  0.9N  NaCl concentration . The incubation  time has not significant effect on yield  of collagen .  The  highest  yield  ( 32.16% )  was  found  by  the  enzymatic  hydrolysis  for  28hr  at  30 ⁰ C  and   (25ml /mg)  of  0.9 N  NaCl  concentration. At lower temperature  (4 ⁰ C) of incubation, the yields  were  less.  The  molecular  weight  of  150kDa  and  250kDa  and  absorbance  at  220 -230nm  of   collagen indicates 99% purity . The FTIR shows the presence of native structure of collagen.  The linear decreasing trend in the relative viscosity of chicken feet collagen was seen  with the  increased  temperature.   Scanning  Electron  Microscopy  ( SEM )  reveals  the  interconnected   network  of  porous  structure  of  collagen.  The  slight  shifting  of  all  the  peaks  of  FTIR  is  the   concrete  evidence  of  interactions  that  occurs  between  the  Polycapralactone  (PCL)   and  the   collagen. The Minimum Inhibitory Concentration ( MIC ) for  Ag -PCL -collagen nanofibers was found to be 0.5 mm at 10 mg/ml for  Ecsherichia coli  and 0.5 mm at 20 mg/ml for  Salmonella  typhimurium .  The combination of silver -collagen -Polycapralactone fibers  could be used as a  bio packaging materials. 0aBioactive compounds. 0aBiopolymers. 0aFunctional foods.1 aAnal, Anil Kumar,eChairperson0 aAthapol Noomhorm,eExamination Committee1 aNguyen, Loc Thai, eExamination committee0 aPisut Koomsap,eExamination Committee2 aHer Majesty Queen{u2019}s Schol arship, eScholarship donor2 aAsian Institute of Technology.tThesis ;vno. no.FB-15-09  3Full-Textuhttp://203.159.5.9/ait-thesis/detail.php?q=B02847