03870nas a2200505 a 4500 20260818144526.0 190829s2018 th uu m rtt 0| a1eng d .b12310220 AIT Thesis no.FB-18-02 Prajapati, Saugat Isolation and characterization of keratinolytic bacteria from chicken farm bed and its application on production of keratin hydrolysates from chicken feather Pathum Thani, Thailand : Asian Institute of Technology, 2018 65 leaves : ill. + 1 online resource Thesis ; no.FB-18-02 A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development Thesis (M.Eng.) - Asian Institute of Technology, 2018 The aim of this research was to isolate a potential keratinolytic bacterial strain from chicken farm bed and use it to produce keratin hydrolysates from chicken feather. In addition, the physicochemical and bioactive properties of the produced keratin hydrolysates was studied. Isolation was carried out by spread plate method in skim milk agar (SMA) plates followed by growing in whole feather broth to study their keratin hydrolyzing activities. As a result, a bacterial strain KB1 was isolated with immense keratin hydrolysis. The BLAST search result of 16s rDNA sequence against the database showed that strain KB1 is 99.78% similar with Bacillus siamensis and Bacillus velezensis. The fermentation process was optimized by using Response surface methodology and Box-Behnken design. The optimum fermentation condition (desirability: 0.976) was found at 40oC, initial pH of 8.84 and 1% (w/v) feather concentration with the production of 252.95 mg/g of soluble protein and 88.19% feather degradation respectively. For feather. The keratin hydrolysate contained 78.45 % protein with 82.36 % digestibility. The IC50value of keratin hydrolysate for DPPH free radical scavenging activity was found to be 0.7 mg/ml and ferric reducing value of 130.41og AAE/ml. Keratin Bacteria Bioactive compounds Biotechnology Anal, Anil Kumar, Chairperson Loc Thai Nguyen, Examination committee Datta, Avishek, Examination Committee Sadiq, Muhammad Bilal, Examination committee Wonnop Visessanguan, Examination committee Thailand (HM Queen), Scholarship donor Asian Institute of Technology. Thesis ; no.FB-18-02 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B04599 .b12310220 mnait m 240925 0 190829 m a m 0 000:001:PDF:b1231022:004346:0:0:0:0:0:0 Click to view Abstract n mnait mnait mnarc mnarc 40 61 20 Barcode : 30050120879357 CREATED : 2019-05-27 RECORD # : i13189128 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : - CREATED : 2019-05-27 RECORD # : i1318913x LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : 30050120955330 CREATED : 2019-08-29 RECORD # : i13214299 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 Barcode : 30050120955306 CREATED : 2019-08-29 RECORD # : i13214317 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 65453 65453 0 0 0 0 MAIN MAIN mnarc 2026-08-18 0 AIT Thesis no.FB-18-02 30050120879357 2026-08-18 14:45:26 1 2026-08-18 40 0 0 0 0 MAIN MAIN mnarc 2026-08-18 0 AIT Thesis no.FB-18-02 2026-08-18 14:45:26 2026-08-18 61 0 0 0 0 MAIN MAIN mnait 2026-08-18 50.00 0 AIT Thesis no.FB-18-02 30050120955330 2026-08-18 14:45:26 1 2026-08-18 20 0 0 0 0 MAIN MAIN mnait 2026-08-18 50.00 0 AIT Thesis no.FB-18-02 30050120955306 2026-08-18 14:45:26 2 2026-08-18 20