TY  - SER
AU  - Prajapati,Saugat
AU  - Anal,Anil Kumar
AU  - Loc Thai Nguyen
AU  - Datta,Avishek
AU  - Sadiq,Muhammad Bilal
AU  - Wonnop Visessanguan,
ED  - Thailand (HM Queen),
TI  - Isolation and characterization of keratinolytic bacteria from chicken farm bed and its application on production of keratin hydrolysates from chicken feather
T2  - Thesis
PY  - 2018///
CY  - Pathum Thani, Thailand
PB  - Asian Institute of Technology
KW  - Keratin
KW  - Bacteria
KW  - Bioactive compounds
KW  - Biotechnology
N1  - A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development; Thesis (M.Eng.) - Asian Institute of Technology, 2018
N2  - The aim of this research was to isolate a potential keratinolytic bacterial strain from chicken farm  bed  and  use  it  to  produce  keratin  hydrolysates  from  chicken  feather.  In  addition,  the physicochemical and bioactive properties of the produced keratin hydrolysates was studied. Isolation was carried out by spread plate method in skim milk agar (SMA) plates followed by growing  in  whole  feather  broth  to  study  their  keratin  hydrolyzing  activities.  As  a  result,  a bacterial strain KB1 was isolated with immense keratin hydrolysis. The BLAST search result of 16s rDNA sequence against the database showed that strain KB1 is 99.78% similar with Bacillus siamensis and Bacillus velezensis. The fermentation process was optimized by using Response  surface  methodology  and  Box-Behnken  design.  The  optimum  fermentation condition  (desirability:  0.976)  was  found  at  40oC,  initial  pH  of  8.84  and  1%  (w/v)  feather concentration  with  the  production  of  252.95  mg/g  of  soluble  protein  and  88.19%  feather degradation respectively. For feather. The keratin hydrolysate contained 78.45 % protein with 82.36 % digestibility. The IC50value of keratin hydrolysate for DPPH free radical scavenging activity was found to be 0.7 mg/ml and ferric reducing value of 130.41og AAE/ml
UR  - http://203.159.5.9/ait-thesis/detail.php?q=B04599
ER  -