TY  - SER
AU  - Singh,Manisha
AU  - Anil Kumar Anal
AU  - Athapol Noomhorm,
AU  - Loc Thai Nguyen
ED  - Asian Development Bank{u2013}Japan Scholarship Program (ADB-JSP), 
TI  - Assessment of bioactive components in dammar extracts and exploration of composite film based on dammar
T2  - Thesis
PY  - 2016///
CY  - Pathum Thani, Thailand
PB  - Asian Institute of Technology
KW  - Bioactive compounds
KW  - Plant bioactive compounds
KW  - Mecidinal plants
N1  - A thesis submitted in partial fulfillment of the requirements for thedegree of Master of ScienceinFood Engineeringand Bioprocess Technology, School of Environment, Resources and Development; Thesis (M. Sc.) - Asian Institute of Technology, 2016
N2  - Plant sourceshave been extensively investigated for bioactive compounds for their potential applications in food, feed, pharmaceutics, biotechnology and medical applications. Dammar, a plantresin,   is   the   secondary   metabolite   secreted   by   many   species   of   family Dipterocarpacae. Though having highpotential bioactive properties and high value, dammar has  still  been  underutilized  and  fewer  investigations  have  been  conducted. Ultrasoundassisted extractionwas applied for the extraction of bioactive constituents from dammar inwhich ethanolwas  used  as  solvent.The effect  of  three  independent  variables;  ethanol concentration,  volume  of  solvent  used  and  extraction  time  on  the  extraction  yield,  total phenolic content, total flavonoid content, antioxidant activity as scavenging activity of 1,1-diphenyl-2-picrylhydrazyl  (DPPH)  radical  and  ferricreducing  antioxidant  power  (FRAP) assay and reducing powerwas examined by response surface methodology (RSM) together with Box-Behnkendesign. The optimized conditionsfor bioactive extraction as predicted by RSM were99.8% ethanol(v/v), 30 ml volume of solvent per gof raw materialsand 40 minof  extractiontime.  The  extraction  yield  at  theseconditionswas  46.40%. Similarly, the optimal ultrasonic-assisted conditionsfor extraction of phenolic, antioxidantand reducing compounds wereestablished as ethanolconcentration of 60%(v/v), 10 ml volume of solvent per  g of  raw  materials and  extraction  time  of60  min. Under  these  conditions,  the  total phenolic  content,  antioxidant  activity  as  %DPPH,  antioxidant  activity  as  FRAP  and reducing power were17.18 mg gallic acid equivalent/ g, 83.97 %, 877.874 omol/g and 23.01ascorbic acid equivalent/ grespectively.Statistically, only concentration of ethanol indicated significant effect on the bioactive properties of extract.In addition, the extractsfrom lowerconcentration of ethanol also exhibited antibacterial property against gram positive bacteria(Streptococcus  agalactiaeand Staphylococcus  aureus). Furthermore, dammar  have  been explored   to   develop the   composite   film   with   gelatin. Thethickness,   water   vapor permeability, tensile strength, elongation at break and color propertiesof different films with varying   proportion   of   dammar   and   gelatin were   evaluated. The   composite   films demonstrated satisfactory  physicochemical  properties  having  probableapplication  in  the field of food and pharmaceutical industries
UR  - http://203.159.5.9/ait-thesis/detail.php?q=B02855
ER  -