05198nas|a2200433 i 4500 20260818173613.0 160501s2000 th uzm rtt 00| a1eng d .b11826058 AIT Diss. no. AS-00-02 Agus Suyanto Potential use of entomopathogenic nematodes for controlling lepidopterous crucifer pest complex in Indonesia Bangkok : Asian Institute of Technology, 2000 126 leaves Dissertation ; no. AS-00-02 A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Technical Science, School of Environment, Resources and Development Thesis (Ph.D.) - Asian Institute of Technology, 2000 The lepidopterous pests are the major constraint in crucifer production in Indonesia. Due to the ineffectiveness of certain control measures, entomopathogenic nematodes may be an alternative to control the pest problem. However, the use of these natural enemies in crucifer crop production in Indonesia is insufficient due to lack of the basic and essential knowledge about them. Therefore, a research, which focused on determining the potential of entomopathogenic nematodes, was conducted in the center of crucifer production in Banjarnegara district, Central Java province, Indonesia. The study was conducted from May 1998 to February 1999. The overall objective of the study was to explore various entomopathogenic nematodes found in the crucifer crop ecosystem and to investigate the feasibility of utilizing the promising nematodes in pest management programs for the lepidopterous crucifer pest complex. More specifically, this study was conducted to search for and identify the nematodes. The possibility to culture and efficacy of the nematodes in the pest management program for lepidopterous crucifer pest complex were also evaluated. A preliminary survey showed that farmers in the survey site commonly cultivated the cabbage (Brassica oleracea L.). There were three major pests, namely, cabbage head caterpillar, Crocidolomia pavonana (F.) (Lepidoptere: Pyralidae); cluster caterpillar, Spodoptera litura (F.) (Lepidoptera: Noctuidae) and diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae). For controlling the pests, most of the farmers always used insecticides based on the schedule. An entomopathogenic nematode, Heterorhabditis sp. (Rhabditida: Heterorhabditidae) has been found in the soil throughout the crucifer are and other agroecosystem around the area. The nematodes could be recovered by using a laboratory and bioassays in the laboratory. The existence of this biological control agent in the andosol soil was less than the regosol soil. It appears from the results that monoxenic culture, which contains 60% beef kidney, 20% beef fat, and 20% tap water, was suitable for Heterorhabditis sp. The highest average yield was more than 20 million nematodes per liter of suspension that could be harvested in the third week. The nematodes was more toxic to the cabbagehead caterpillar (C. pavonana) and the cluster caterpillar (S. litura) than to the diamondback moth (P. xylostella). Effective concentration on the cabbagehead caterpillar (C. pavonana) and the cluster caterpillar (S. litura) was 300 nematodes/ml of water. However, a satisfactory level of control on the diamondback moth (P. xylostella) could not be determined yet, and thus needs further investigation. The results also showed that Heterorhabditis sp. could substitute several synthetic insecticides. When compared with the insecticides, the effectiveness of Heterorhabditis sp. against the lepidopterous pests was comparable with a synthetic pyrethroid (cyhalotrin), some carbamate insecticides (BPMC and carbosulfan) and some organophosphorous (profenofos and methidathion). Moreover, Heterorhabditis sp. was more effective than the microbial insecticide, Bacillus thuringiesis Berliner. Cruciferae Diseases and pests Indonesia Pests Biological control Indonesia Shivakoti, Ganesh P., Chairperson Banpot Napompeth, Co-Chairperson Salokhe, Vilas M., Examination Committee Apisit Eiumnoh, Examination Committee Higher Education Project, Scholarship Donor ADB Loan No. 1253, Scholarship Donor INO, Scholarship Donor Asian Institute of Technology. Dissertation ; no. AS-00-02 Full-Text http://203.159.5.9/ait-thesis/detail.php?q=B00011 .b11826058 mnait u 240402 0 010515 m a u 0 mnait mnarc 22 40 Barcode : 30050120563357 CREATED : 2001-05-16 RECORD # : i12243504 LPATRON : 1015151 LCHKIN : 2019-03-27 # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 6 TOT RENEW : 11 Barcode : 30050160038393 CREATED : 2017-04-04 RECORD # : i13073230 LPATRON : 0 LCHKIN : - # RENEWALS : 0 # OVERDUE : 0 IUSE3 : 0 TOT CHKOUT : 0 TOT RENEW : 0 90022 90022 0 0 0 0 MAIN MAIN mnait 2026-08-18 50.00 0 AIT Diss. no. AS-00-02 30050120563357 2026-08-18 17:36:13 3 2026-08-18 22 0 0 0 0 MAIN MAIN mnarc 2026-08-18 50.00 0 AIT Diss. no. AS-00-02 30050160038393 2026-08-18 17:36:13 1 2026-08-18 40