000 04289nas a2200409 a 4500
005 20260818213659.0
008 230214s2023 th uu m rtt 0| a1eng
035 _a.b12399437
099 9 _aAIT Thesis no.FI-23-01
100 1 _aBayasgalan, Namuunzul
245 1 0 _aSelf assembled nanoparticles of peptides from pea (pisum sativum) and rice (oryza sativa) to enhance their functionality
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2023
300 _a95 leaves :
_bill.
490 1 _aThesis ;
_vno.FI-23-01
500 _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Innovation, Nutrition and Health, School of Environment, Resources and Development
502 _aThesis (M. Sc.) - Asian Institute of Technology, 2023
520 _aThis study focuses on the fabrication of self-assembled bioactive peptides to nanoparticles using peptides and proteins obtained from Peas (Pisum sativum) and Rice (Oryza sativa) to enhance their functionality. The goal of the research is to create a new method for using these plant-based materials in applications to combat multidrug and antibiotic-resistant drugs used in the food system. Antibiotic-resistant bacteria are immune to the effects of commonly used antibiotics, making it difficult to treat infections caused by these bacteria. The study investigates the potential of these peptide and nanoparticles in terms of their physical and chemical properties, as well as their ability to improve the functionality of the proteins and peptides they are made from, and conducting analysis and characterization of the antimicrobial and antioxidant activity of the bio-nanoparticles formed. The study found that the nanoparticles denoted as PPN2 were effective in inhibiting the growth of S.aureus and E.coli, with a zone of inhibition of initially at 11 ± 0.2 to 16.4 ± 0.1, MIC of 75mg/ml, a particle size of d = ~330 nm and zeta potential of -26.23 ± 0.88, obtained under optimal conditions. Bioactive properties were confirmed by conduction agar well and disc diffusion method, broth macrodilution, antibiogram of microbes used, and DPPH assay. Investigation of the properties and potential applications of plant-based nanoparticles (PPN) derived from Pea (Pisum sativum) peptides (PP) and Rice (Oryza sativa) peptides (RP). The research focuses on six different nanoparticles: PPN1, PPN2, PPN3, RPN1, RPN2, and RPN3, which were fabricated using different heat treatments at 45oC, 65oC, and 85oC, respectively. The physical study of fabricating peptide nanoparticles was further confirmed for their morphological study conducted by SDS-PAGE and scanning electron microscope (SEM) and with SDS-PAGE. The chemical study was confirmed for the effects of thermal treatment to the nanoparticles presented by Fourier transform infrared spectroscopy (FTIR). The study also explores the issue of natural peptides that lack efficient antimicrobial peptides in commercial applications and often present with instability with drawback benefits. Thus, the potential of these fabricated nanoparticles in applications for disinfectants provides insight into the potential uses of plant-based nanoparticles in a wide range of fields.
650 0 _aNanoparticles
650 0 _aPeptides
_xBiotechnology
650 0 _aAntioxidants
_xBiotechnology
700 1 _aAnal, Anil Kumar,
_eChairperson
700 0 _aLoc Thai Nguyen,
_eExamination committee
700 0 _aHa Thanh Dong,
_eExamination committee
710 2 _aGovernment of Korea,
_eScholarship Donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno.FI-23-01
856 4 0 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B18283
907 _a.b12399437
_bmnait
_cm
902 _a250421
998 _b0
_c230214
_dm
_ea
_fm
_g0
945 _lmnarc
945 _lmnarc
942 _c67
942 _c40
909 _aBarcode : -
_bCREATED : 2023-10-02
_cRECORD # : i13431821
_dLPATRON : 0
_eLCHKIN : -
_f# RENEWALS : 0
_g# OVERDUE : 0
_hIUSE3 : 0
_iTOT CHKOUT : 0
_jTOT RENEW : 0
909 _aBarcode : 30050121041767
_bCREATED : 2023-02-13
_cRECORD # : i13431985
_dLPATRON : 0
_eLCHKIN : -
_f# RENEWALS : 0
_g# OVERDUE : 0
_hIUSE3 : 0
_iTOT CHKOUT : 0
_jTOT RENEW : 0
999 _c105649
_d105649