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008 980316s1997 th uu m rtt 000 a eng d
035 _a.b11699462
099 9 _aAIT Thesis no.AS-97-14
100 1 _aSheng, Haiqing
245 1 0 _aComparison of the genetic structure of four tilapia strains using mtDNA analyses
260 _aBangkok :
_bAsian Institute of Technology,
_c1997
300 _a52 leaves
490 1 _aThesis ;
_vno. AS-97-14
500 _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
502 _aThesis (M.Sc.) - Asian Institute of Technology, 1997
520 _aMitochondrial DNA (mtDNA) was isolated from four commercial strains of tilapia (Orechromis niloticus): AIT, CHITRALADA, GIFT and UDORN strain hold in the Agricultural and Aquatic System Program (AASP) at AIT. Restriction endonuclease analysis of mtDNA has been carried out to compare genetic structure and characterize differentiation in the four strains. The molecular size of mtDNA of these strains was close to 14500 base pairs. The mtDNA was digested by six hexanucleotide restriction enzymes and analyzed by agarose gel electrophoresis with ethidium bromide staining to reveal the restriction patterns of mtDNA. The results show that the fragment patterns produced by two enzymes (Apa I and Dra I) could be used as genetic markers for identification purposes among the four strains of Orechromis niloticus.
650 0 _aNile tilapia
650 0 _aFishes
_xGenetics
700 1 _aStevens, Willem Frans,
_eChairperson
700 1 _aYakupitiyage, Amararatne,
_eExamination Committee
700 1 _aLittle, David C.,
_eExamination Committee
710 2 _aThe Royal Netherlands Government,
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno. AS-97-14
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B13507
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