| 000 | 03654nas|a2200409 a 4500 | ||
|---|---|---|---|
| 005 | 20260818085159.0 | ||
| 008 | 060911s2006 th uu|m rtt 0| a1eng d | ||
| 035 | _a.b11992669 | ||
| 099 | 9 | _aAIT Thesis no.EV-06-1 | |
| 100 | 0 | _aAnchalee Charoenwuttitam | |
| 245 | 1 | 0 | _aCopper resistant mechanism in Agrobacterium tumefaciens NTL4 |
| 260 |
_aPathum Thani, Thailand : _bAsian Institute of Technology, _c2006 |
||
| 300 |
_a86 leaves : _bill. |
||
| 490 | 1 |
_aThesis ; _vno. EV-06-1 |
|
| 500 | _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science Inter-University Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development | ||
| 502 | _aThesis (M.Sc.) - Asian Institute of Technology, 2006 | ||
| 520 | _aAgrohacterum tumefaciens possesses multiple system to ensure proper copper handling under varying environmental condition. Searching A. tumefaciens C58 genome revealed that the bacteria had three loci (two on circular and one on linear chromosome) contained genes involved in copper resistance. Here, three genes, namely cueR (Atu0938), copA (Atu0937) and CueO (Atu3991) from two loci were functionally evaluated in term of copper resistance. CueR is transcriptional regulator in a MerR family while CopA and CueO are copper transporting P-type APpase and multicopper oxidase, respectively. A. tumefaciens cueR, copA and cueO. Mutants were constructed by gene knockout using pKNOCK system. cueO mutant was sensitive to Cu (II) and Cd (II) as tested by inhibition zone assays however all the MIC values against Cu (II) in all the mutant was reduced comparing to the wild type's level. The altered phenotypes could be restored by a plasmidborne expression of individual gene. Northern blot analysis revealed that the expression of cueO could be induced only by Cu (II) in an CueR-independent manner. Promoter analysis using lacZ fusion revealed that copA expression was controlled by CueR and could be hypothesized that when A. tumefaciens exposes to increase level of copper (as well as Ag) ions. Two transcription regulators, CueR and the unknown regulator, respond to this change by promote transcription of copA encoding efflux protein and cueO encode multicopper oxidase. High expression of these two protein makes A. tumefaciens more resistant to copper | ||
| 650 | 0 |
_aCopper _xEnvironmental aspects |
|
| 700 | 1 |
_a Paiboon Vattanaviboon, _eChairperson |
|
| 700 | 0 |
_aSkorn Mongkolsuk, _eExamination Committee |
|
| 700 | 0 |
_aPreeda Pakpian, _eExamination Committee |
|
| 710 | 2 |
_aChulabhom Research Institute I Mahidol University I Asian Institute of Technology (CRI-MU-AIT), _eScholarship donor |
|
| 810 | 2 |
_aAsian Institute of Technology. _tThesis ; _vno. EV-06-1 |
|
| 856 |
_3Full-Text _uhttp://203.159.5.9/ait-thesis/detail.php?q=B10590 |
||
| 907 |
_a.b11992669 _bmnait _cu |
||
| 902 | _a240401 | ||
| 998 |
_b0 _c060911 _dm _ea _fu _g0 |
||
| 945 | _lmnait | ||
| 945 | _lmnait | ||
| 945 | _lmnarc | ||
| 942 | _c20 | ||
| 942 | _c40 | ||
| 909 |
_aBarcode : 30050120737217 _bCREATED : 2005-04-14 _cRECORD # : i12495712 _dLPATRON : 1020210 _eLCHKIN : 2008-04-02 _f# RENEWALS : 0 _g# OVERDUE : 0 _hIUSE3 : 2 _iTOT CHKOUT : 5 _jTOT RENEW : 0 |
||
| 909 |
_aBarcode : 30050120737183 _bCREATED : 2005-04-14 _cRECORD # : i12495724 _dLPATRON : 1018265 _eLCHKIN : 2006-01-11 _f# RENEWALS : 0 _g# OVERDUE : 0 _hIUSE3 : 0 _iTOT CHKOUT : 2 _jTOT RENEW : 2 |
||
| 909 |
_aBarcode : 30050160050422 _bCREATED : 2016-05-25 _cRECORD # : i12991697 _dLPATRON : 0 _eLCHKIN : - _f# RENEWALS : 0 _g# OVERDUE : 0 _hIUSE3 : 0 _iTOT CHKOUT : 0 _jTOT RENEW : 0 |
||
| 999 |
_c23431 _d23431 |
||