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035 _a.b12398275
099 9 _aAIT Thesis no.ISE-22-10
100 0 _aNapatsakorn Kamkrua
245 1 0 _aDesign and fabrication of aptamer-based SERS chips for selective detection of paraquat herbicide residues in water and soil
260 _aPathum Thani :
_bAsian Institute of Technology,
_c2022
300 _a71 p. :
_bill.
490 1 _aThesis ;
_vno. ISE-22-10
502 _aThesis (M. Eng.) - Asian Institute of Technology, 2022
500 _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Nanotechnology
520 _aA common herbicide such as paraquat has been countlessly applied in agriculture, and its residues are left in water and soil. Paraquat toxicity highly damages the body, which can be lethal in a later stage. Thus, paraquat detection is important and several techniques have been developed in recent years. Raman spectroscopy facilitates herbicide detection in rapid and non-destructive ways. Furthermore, surface-enhanced Raman spectroscopy (SERS) and bioreceptors are commonly utilized to enhance the sensitivity and selectivity of molecular structure detection, respectively. This study focuses on optimizing SERS-active substrates to improve the selectivity and rapidity of paraquat detection. This work also aimed to develop the combinations of SERS and biosensors for paraquat detection by functionalizing metallic nanostructures with an aptamer that is specific to the paraquat molecule via Raman spectroscopy. Soil and water samples were used for the evaluation of optimized SERS-active substrates. The sensitivity, selectivity, and limit of detection were investigated by Raman measurement. This study optimized the ONSPEC-Prime SERS chip (AgNRs) for paraquat detection in water and soil samples. Without extraction protocol, the chip provided a sensitivity of 638.96 a.u./æM and a detection limit (LOD) of 0.1 æM for the water sample. However, in the soil samples, paraquat extraction protocol with alumina absorbent is required. Furthermore, the aptamer-based SERS chip was successfully fabricated with a sensitivity of 609.15 a.u./æM and LOD of 0.35 æM using 0.1 æM of aptamer immobilized on the ONSPEC-Lite SERS chip (AuNPs).
650 0 _aRaman spectroscopy
650 0 _aBiosensors
700 1 _aBora, Tanujjal,
_eChairperson
700 0 _aNoppadon Nuntawong,
_eCo-Chairperson
700 1 _aRicco, Raffaele,
_eExamination committee
700 0 _aLoc Thai Nguyen,
_eExamination committee
710 2 _aRoyal Thai Government Scholarships ,
_eScholarship Donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno. ISE-22-10
856 4 0 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B18184
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_bCREATED : 2023-01-02
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