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035 _a.b12096404
099 9 _aAIT Diss. no.EV-10-06
100 0 _aTanvisith Charoenying
245 1 0 _aEffects of pesticides on the non-neuronal lymphocytic cholinergic system
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2010
300 _a118 p. :
_bill.
490 1 _aDissertation ;
_vno. EV-10-06
500 _aA dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management
502 _aThesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidal University, 2010
520 _a Concerns over adverse effects of pesticide -induced immunotoxic effects are heightened; however , knowledge on this area is relatively still limited . Subsequent to the discovery of non- neuronal cholinergic system in lymphocytes; suggesting that it might be susceptible to pesticides exposure. Pesticides namely, permethrin (PM), deltamethrin (DM), pa raquat (PQ), and paraoxon (PO) were selected to study for their immuno toxic effects. PM and PQ were studied in mice challenged with Plasmodium berghei . The resul ts showed that survival time of mice was significantly reduced and parasitemia percentages of PM- treated groups were slightly higher than control group as a result of prior oral exposure to PM for 14 days and during malaria infection . In contrast, PQ did not increase host susceptibility to Plasmodium infection in PQ -treated mice. The in vitro study showed that all selected pesticides reduced viability of SH -SY5Y, human neuroblastoma and MOLT -3, human leukemic T -lymphocytes cells. The effects of PO were fur ther investigated in SH -SY5Y and MOLT -3 cells. PO were able to induce change s of cholinergic system in both cells in a more or less similar response profile demonstrated by over -expression of AChE, a marker of apoptosis, alterations of M1, M2, and M3 mAChR s mRNA or protein s, and activation of the 2early responsive gene3 c -Fos gene expression. Moreover, the PO -induced activation N -AChE and c -Fos in both neuronal and non- neuronal cells were able to p artially be blocked by atropine indicating that mAChRs activ ation is partially involved in the PO -mediated cellular responses. Taken together, the results suggest that certain neurotoxic pesticides can elicit neurotoxic and immunotoxic effects. The similarity of the response profile in both neuronal and non- neuronal cells suggest that biological functions of such components are, more or less, alike. This present study provides initial and additional information in whic h non- neuronal lymphocytic muscarinic receptors are possible potential targets for pesticides -indu ced immunotoxic effects. In addition the present study also strengthens the belief of using lymphocytes as a surrogate marker for evaluating neurotoxic condition as the consequence of certain pesticides exposure.
650 0 _aPesticides
_xPhysiological effect
650 0 _aMice
_xEffect of pesticides on
700 0 _aJutamaad Satayavivad,
_eChairperson
700 0 _aPreeda Pakpian,
_eExamination Committee
700 0 _aApinya Thiantanawat,
_eExamination committee
700 0 _aSansanee Chaiyaroj,
_eExamination committee
710 2 _aChulabhorn Research Institute,
_eScholarship donor
710 2 _aMahidol University,
_eScholarship donor
710 2 _aAsian Institute of Technology Fellowship,
_eScholarship donor
710 2 _aRoyal Thai Government,
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tDissertation ;
_vno. EV-10-06
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B00562
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_tAbstract-AIT Diss. no.EV-10-06
_vn
945 _lmnait
945 _lmnarc
942 _c22
942 _c40
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