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| 008 | 180911s2016 th uu m rtt 0| a1eng d | ||
| 035 | _a.b12206441 | ||
| 099 | 9 | _aAIT Thesis no.FB-16-07 | |
| 100 | 1 | _aAzad, Mirza Rajiv | |
| 245 | 1 | 0 | _aEvaluation of encapsulated Lactobacillus bulgaricus for their viability in feed pellets and simulated gastrointestinal conditions of poultry |
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_aPathum Thani, Thailand : _bAsian Institute of Technology, _c2016 |
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_a69 leaves : _bill. + _e1 online resource |
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| 490 | 1 |
_aThesis ; _vno.FB-16-07 |
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| 500 | _a A thesis submitted in partial fulfillment of the requirements for the degree of Ma ster of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development | ||
| 502 | _aThesis (M. Sc.) - Asian Institute of Technology, 2016 | ||
| 520 | _aThe greatest hurdles that probiotic bacteria must overcome during feed processing, storage and in the upper gastrointestinal tract are acid and th e presence of bile. T he main purpose of this study was to develop a novel encapsulation techn i que to enhance the stability of thermal and acidic protection of probiotic bacteria. T hree encapsulating bio materials were considered as good potential carrier material s for encapsulating the L actobacillus strain namely Lactobacillus bulgaricus to protect from adverse environmental conditions. Capsules of alginate - whey protein isolate and alginate - resistant starch w ere produced at various concentrations and analyzed for thermal stress to be incorporated in poultry feed . A lginate - resistant starch capsules pr oved better viability at high processing temperature up to 90°C for 90 seconds . In this study , a disaccharide trehalose was also used to incorporate with alginate - resistant starch to increase the resistance to high temperature . Moreover, t he acid tolerance of the encapsulated cells was compared with the free cells in an in vitro gastric model and a significantly higher survival was recorded for encapsulated cells. A similar trend was observed in the presence of bile salts. Physical p roperties of alginate - re sistant starch capsules were also observed. The mean diameter of wet and freeze dried capsules of the combination of 2% (w/v) sodium alginate, 4% (w/v) resistant starch and 1% (w/v) trehalose were found to be in an acceptable range. The particles size had diameters of 1.71 ± 0.15 and 1.41 ± 0.12 mm for wet and freeze dried capsules , respectively. L. bulgaricus encapsulated with alginate - resistant starch - trehalose had higher ability of thermal protection at 90°C; viability was 2.84 Log CFU/g up to 90s for w et capsules than non - encapsulated bacteria l cell. A fter incubation in poultry simulated gastric fluid the viable count reduced from 8.51 to 5.20 log CFU/g for wet capsules whereas free cells were not detectable after 30 min of exposure. There was almost no detrimental effect of bile on encapsulated cells, 1.06 and 1.37 log CFU/g reductions in case of wet capsules for 1% a nd 2% (w/v) bile, respectively. The encapsulated L. bulgaricus released 7.99 log CFU/g viable cells in intestinal c olonic pH 6.8 within 2 h . Storage of encapsulated wet capsules at 37°C proved to be more detrimental to the entrapped cells than at 4°C. | ||
| 650 | 0 | _aLactobacillus casei | |
| 650 | 0 | _aGastrointestinal system | |
| 700 | 1 |
_aAnal, Anil Kumar, _eChairperson |
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| 700 | 1 |
_aNguyen, Loc Thai, _eExamination committee |
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| 700 | 0 |
_aAthapol Noomhorm, _eExamination Committee |
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_aC.P. Bangladesh Co., Ltd., _eScholarship donor |
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| 810 | 2 |
_aAsian Institute of Technology. _tThesis ; _vno. no.FB-16-07 |
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_3Full-Text _uhttp://203.159.5.9/ait-thesis/detail.php?q=B02856 |
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_a000:001:PDF:b1220644:003850:0:0:0:0:0:0 _tClick to view Abstract _vn |
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