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008 190322s2015 th uu m rtt 0| a1eng d
035 _a.b1229990x
099 9 _aAIT Thesis no.FB-15-09
100 1 _aDhakal, Damodar
245 1 0 _aExtraction and characterization of collagen to develop biopolymeric fiber from chicken feet.
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2015
300 _a83 leaves :
_bill. +
_e1 online resource
490 1 _aThesis ;
_vno.FB-15-09
500 _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
502 _aThesis (M. Sc.) - Asian Institute of Technology, 2015
520 _a Collagen was extracted sequencially from the chicken feet using papain, acetic acid and NaCl solution. The optimum condition was found to be 30 ⁰ C temperature treatment and ( 25 ml/mg ) 0.9N NaCl concentration . The incubation time has not significant effect on yield of collagen . The highest yield ( 32.16% ) was found by the enzymatic hydrolysis for 28hr at 30 ⁰ C and (25ml /mg) of 0.9 N NaCl concentration. At lower temperature (4 ⁰ C) of incubation, the yields were less. The molecular weight of 150kDa and 250kDa and absorbance at 220 -230nm of collagen indicates 99% purity . The FTIR shows the presence of native structure of collagen. The linear decreasing trend in the relative viscosity of chicken feet collagen was seen with the increased temperature. Scanning Electron Microscopy ( SEM ) reveals the interconnected network of porous structure of collagen. The slight shifting of all the peaks of FTIR is the concrete evidence of interactions that occurs between the Polycapralactone (PCL) and the collagen. The Minimum Inhibitory Concentration ( MIC ) for Ag -PCL -collagen nanofibers was found to be 0.5 mm at 10 mg/ml for Ecsherichia coli and 0.5 mm at 20 mg/ml for Salmonella typhimurium . The combination of silver -collagen -Polycapralactone fibers could be used as a bio packaging materials.
650 0 _aBioactive compounds.
650 0 _aBiopolymers.
650 0 _aFunctional foods.
700 1 _aAnal, Anil Kumar,
_eChairperson
700 0 _aAthapol Noomhorm,
_eExamination Committee
700 1 _aNguyen, Loc Thai,
_eExamination committee
700 0 _aPisut Koomsap,
_eExamination Committee
710 2 _aHer Majesty Queen{u2019}s Schol arship,
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno. no.FB-15-09
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B02847
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