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035 _a.b12310025
099 _aAIT Thesis no.FB-17-01
100 1 _aShrestha, Pratiksha
245 1 0 _aExtraction of crystalline nanocellulose from banana pseudostems and its utilization in developing bio-nanocomposite film
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2017
300 _a78 leaves :
_bill. +
_e1 online resource
490 1 _aThesis ;
_vno.FB-17-01
500 _a A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
502 _aThesis (M. Sc.) - Asian Institute of Technology, 2017
520 _a Lignocellulosic agricultural residue is one of the cheapest source of cellulose based biomaterial. Myriad of research have been conducted on extraction of crystalline nanocellulose and its characterization but only little is known about its drug binding ch emistry and chemical modifications limiting its utilization in biomedical applications. In this work crystalline nanocellulose (CNC) was isolated from banana pseudostem waste by acid hydrolysis method and tetracycline (TC) loaded nanocomposite was develope d by crosslinking with Butane tetra carboxylic acid ( BTCA ) and chitosan. Thus obtained CNCs were found to have have nanoscopic dimension (18.79±5.30 nm diameter and 202.12±37.43 nm length) and exhibited high degree of crystallinity (81.67%). Morpholog ical study and chemical characterization of CNCs were performed by Scanning Electron Microscopy (SEM), Fourier Transmission Infra - Red (FT - IR) spectroscopy , Thermo gravimetric analysis (TGA) and X - Ray Diffraction (XRD). Nanocomposites were prepared by four diffe rent modification methods by crosslinking with BTCA and varying concentration of TC and chitosa n. Films (0.18 ± 0.01 mm) prepared by solvent evaporation method were casted in acrylic plates and film morphology, mechanical properties and thermal behavior w ere studied. In vitro release of TC was carried in citrate buffer (pH 5.7) at incubating condition (37 C̊) with constant stirring (100 rpm) and sustained release of up to 16 h was observed with TC loaded nanocomposite film with modification method 3. Antiba cterial propert ies of nanocomposite film against Gram - positive ( Staphylococcus aureus ) and Gram - negative ( Escherichia coli ) was investigated. Nanocomposite film (25 mm diameter) with modification method 3 showed highest zone of inhibition for both E. coli (36.3 ± 0.6 mm) and Staph. aureus (39.3 ± 0.6 mm) with TC drug loading (1205 og) and crosslinking with chitosan. The obtained nanocomposite can potentially be applied in biomedical field for efficient drug delivery and developing transdermal patches. Findi ng of the present study reveals economic production of biomaterial for medical applications utilizing lignocellulosic waste.
650 0 _aNanocomposites (Materials)
650 0 _aCellulose
_xBiotechnology
650 0 _aCrystalline polymers
700 1 _aAnal, Anil Kumar,
_eChairperson
700 1 _aNguyen, Loc Thai,
_eExamination committee
700 1 _aSadiq, Muhammad Bilal,
_eExamination committee
710 2 _aThailand (HM Queen),
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno. no.FB-17-01
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B02860
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