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008 170399s1993 th uzm rtt 00| a1eng d
035 _a.b10078903
099 9 _aAIT Thesis no.EV-93-06
100 1 _aCheema, Muhammad Javaid
245 1 0 _aAnaerobic degradation of chlorophenols
260 _aBangkok :
_bAsian Institute of Technology,
_c1993
300 _a133 leaves +
_e1 online resource
490 1 _aThesis ;
_vno. EV-93-06
500 _aA thesis submitted in partial fulfillment of the requirements for the Degree of Master of Engineering
502 _aThesis (M.Eng.) - Asian Institute of Technology, 1993
520 _aTwo lab scale anaerobic filters (AFs) were operated to investigate the potential of AFs for the treatment of chlorophenolic wastewater. With AF1, a substrate loading rate of 150 mg/l/day (2-MCP) was possible and with AF2, 20 mg/I/day (2,4,6-TCP) was completely dechlorinated, but major intermediate degradation product, 4-MCP was virtually persistent. About 91 % of total influent TCP organic carbon was converted to 4-MCP. And AF2, community did not have species that could degrade 4-MCP. TCP follow pathways i. e, TCP-> 2,4-DCP--> 4-MCP and 2,4-DCP --> 2-MCP -->phenol. Very less concentration of 2- MCP could be detected in the reactor effluent. The TOC reduction was about 98 % , with mass balance 82 % , and 18 % of the carbon was being utilized for biomass production. In both AFs effluent pH increased (6.8 to 7.9),due to consumption of hydrogen ions, while _reduction of sulfate to H2S and Hs·. SEM study of reactor sludge, fed with 2-MCP showed the dominancy of Methanothrix and Desulfovibrio spp. Concentration of maximum influent possible chloride ions and actual effluent concentration were same, which confirmed that the chlorophenols reduction was due to the biodegradation mechanism, through reductive dechlorination but not due to volatilization, or some abiotic mechanism. Addition of molybdate (20 mM), a specific inhibitor of Sulfate Reducing Bacteria (SRB) completely inhibited the dechlorination of 2-MCP and ring cleavage of phenol did not occur also. But degradation of 2-MCP also completely stopped in the MPBs inhibited enrichment with the addition of 0.5 mM chloroform. So it can be inferred that 2-MCP degradation was not only due to SRBs, but due to syntrophic association with MPBs. Relative disappearance of monochlorophenols isomers, in the TCP acclimated community, was in this order: ortho > meta >para, demonstrated that rate of dechlorination of different monochlorophenol isomers was related to position of chlorine substituent on the benzene ring. Presence of glucose as cosubstrate stimulated, dechlorination of chlorophenols.
650 0 _aSewage
_xPurification
_xPhenol removal
700 1 _aTanaka, Shuzo,
_eChairperson
700 0 _aChongrak Polprasert,
_eExamination Committee
700 1 _aVisvanathan, C.,
_eExamination Committee
710 2 _aGovernment of Denmark,
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno. EV-93-06
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B16207
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