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035			_a.b1191340x
099		9	_aAIT Thesis no.EV-07-48
100	0		_aSirikan Nawapan
245	1	0	_aFunctional and expression analyses of cop operon required for copper resistance in Agrobacterium tumefaciens
260			_aPathum Thani, Thailand : _bAsian Institute of Technology, _c2007
300			_a57 leaves : _bill.
490	1		_aThesis ; _vno. EV-07-48
500			_aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management
502			_aThesis (M.Sc.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2007
520			_aThe cop operon of Agrobacterium tumefaciens involving in copper resistance is consisted of three genes that encode CPx-type copper ATPase, CopA, a repressor, CopR and a copper chaperone, CopZ. The expression of cop operon is induced by the presence of copper and silver thereby protecting A. trmrefaciens from particular metal ion toxicity. Primer extension analysis defined the consensus sequence 5'CTTCCCATnATGGGAAG3' as the repressor binding clement. Using in vivo copARZ promoter analysis, it was demonstrated that copA expression is regulated by copR while CopZ copper chaperone played no role on the function of CopR to sensing copper. Gene disruption of copA and copZ by using the suicide plasmid pKNOCK caused reduction of bacterial ability to cope with copper and silver toxicity. Unfortunately, such reduction could not be complemented by a trans expression of copA or copZ respectively resulting from the polar effect from inactivation of one gene on the expression of other genes in the operon. Taken together, the results suggest that under copper-limiting condition CopR binds to the copARZ promoter and represses the expression of the operon. In high copper, CopR is activated to an active form of transcription activator thereby, enhancing copARZ transcript. A copper chaperone, CopZ then binds excess free Cu(I) and transfers to CopA copper export to detoxify copper
650		0	_aAgrobacterium tumefaciens
650		0	_aCopper _xToxicity testing
700	0		_aPaiboon Vattanaviboon., _eChairperson
700	0		_aSkorn Mongkolsuk, _eExamination Committee
700	0		_aPreeda Pakpian, _eExamination Committee
710	2		_aChulabhom Research Institute, _eScholarship Donor
710	2		_aMahidol University, _eScholarship Donor
710	2		_aAsian Institute of Technology, _eScholarship Donor
810	2		_aAsian Institute of Technology. _tThesis ; _vno. EV-07-48
856			_3Full-Text _u http://203.159.5.9/ait-thesis/detail.php?q=B11006
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