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035 _a.b12310220
099 9 _aAIT Thesis no.FB-18-02
100 1 _aPrajapati, Saugat
245 1 0 _aIsolation and characterization of keratinolytic bacteria from chicken farm bed and its application on production of keratin hydrolysates from chicken feather
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2018
300 _a65 leaves :
_bill. +
_e1 online resource
490 1 _aThesis ;
_vno.FB-18-02
500 _aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
502 _aThesis (M.Eng.) - Asian Institute of Technology, 2018
520 _aThe aim of this research was to isolate a potential keratinolytic bacterial strain from chicken farm bed and use it to produce keratin hydrolysates from chicken feather. In addition, the physicochemical and bioactive properties of the produced keratin hydrolysates was studied. Isolation was carried out by spread plate method in skim milk agar (SMA) plates followed by growing in whole feather broth to study their keratin hydrolyzing activities. As a result, a bacterial strain KB1 was isolated with immense keratin hydrolysis. The BLAST search result of 16s rDNA sequence against the database showed that strain KB1 is 99.78% similar with Bacillus siamensis and Bacillus velezensis. The fermentation process was optimized by using Response surface methodology and Box-Behnken design. The optimum fermentation condition (desirability: 0.976) was found at 40oC, initial pH of 8.84 and 1% (w/v) feather concentration with the production of 252.95 mg/g of soluble protein and 88.19% feather degradation respectively. For feather. The keratin hydrolysate contained 78.45 % protein with 82.36 % digestibility. The IC50value of keratin hydrolysate for DPPH free radical scavenging activity was found to be 0.7 mg/ml and ferric reducing value of 130.41og AAE/ml.
650 0 _aKeratin
650 0 _aBacteria
650 0 _aBioactive compounds
_xBiotechnology
700 1 _aAnal, Anil Kumar,
_eChairperson
700 1 _aLoc Thai Nguyen,
_eExamination committee
700 1 _aDatta, Avishek,
_eExamination Committee
700 1 _aSadiq, Muhammad Bilal,
_eExamination committee
700 0 _aWonnop Visessanguan,
_eExamination committee
710 2 _aThailand (HM Queen),
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno.FB-18-02
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B04599
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_tClick to view Abstract
_vn
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