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035 _a.b1231030x
099 _aAIT Thesis no.FB-18-07
100 0 _aSuwan Panjanapongchai
245 1 0 _aIsolation, molecular identification and microencapsulation of microorganisms in biofertillzer to enhance their functionality
260 _aPathum Thani, Thailand :
_bAsian Institute of Technology,
_c2018
300 _a51 leaves :
_bill. +
_e1 online resource
490 1 _aThesis ;
_vno.FB-18-07
500 _aA Thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
502 _aThesis (M.Sc.) - Asian Institute of Technology, 2018
520 _aApplication of chemical fertilizers has promoted dramatically for increasing of productivity of food in the world and this lead to increasing of application chemical fertilizers in worldwide. On the other hand, that impact environment due to excessive of usage chemical fertilizers. Moreover, synthetic chemical pesticides decrease quality of soil. Biofertilizers are alternative fertilizers and necessary components integration of nutrients management. These Biofertilizers would be the key to increase yield of crop, sustainability of soils and are ecofriendly and effectiveness of cost for farmer. The limitation of biofertilizers are taking long time to obtain clearly result because the microorganism from soil has competition with biofertilizers. In this study, commercial liquid biofertilizers were explored to isolate the microorganisms namely, Deinococcus wulumuqiensis WT/1A, Micrococcus yunnanensis TSA9, Pseudomonas stutzeri DSM 5190, Micoccus yunnansis antCr86, Staphylococcus arlettae PMN and Bacillus aryabhattai B8W22(T) by sequencing of 16s rDNA. Those microorganisms were further characterized for morphology and biochemical test. The waste water containing high amount of carbon source and nitrogen source such as amino acid from monosodium glutamate production factory was used as medium to grow the microorganisms. The maximum number of microorganisms found was 5{u00D7}109 cfu/ml in NB while liquid biofertilizers fermented by monosodium glutamate waste-water were 1.02{u00D7}108 cfu/ml. In addition, the specific growth rate of biofertilizers fermented by nutrient was double to monosodium glutamate waste-water. In this study, using difference coagulation fluid and post-coagulate fluid were used for multilayer beads to obtain the highest entrapment efficiency. Finally, the control release of microencapsulation was measured by inoculation the beads into soil. As a result, the single-layer beads release microorganism in 4 hours and remain till 12 hours while multilayer bead release in 24 hours and remain further 24 hours.
650 0 _aBiofertilizers
650 0 _aMicroencapsulation
700 1 _aAnal, Anil Kumar,
_eChairperson
700 1 _aLoc Thai Nguyen,
_eExamination committee
700 1 _aDatta, Avishek,
_eExamination Committee
700 1 _aSadiq, Muhammad Bilal,
_eExamination committee
710 2 _aRoyal Thai Government Fellowship,
_eScholarship donor
810 2 _aAsian Institute of Technology.
_tThesis ;
_vno.FB-18-07
856 _3Full-Text
_uhttp://203.159.5.9/ait-thesis/detail.php?q=B02871
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_vn
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909 _aBarcode : 30050120955231
_bCREATED : 2019-04-09
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