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005			20260817163003.0
008			060712s2005 th uu|m rtt 0| a1eng d
035			_a.b11991598
099		9	_aAIT Thesis no.FB-05-21
100	0		_aThan Than Sein
245	1	0	_aApplication of Trichoderma harzianum BCC5828 and N- acetylglucosaminidase enzyme as biocontrol agents
260			_aPathum Thani, Thailand : _bAsian Institute of Technology, _c2005
300			_a55 leaves : _bill. + _e1 online resource
490	1		_aThesis ; _vno. FB-05-21
500			_aA thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development
502			_aThesis (M.Eng.) - Asian Institute of Technology, 2005
520			_aTrichoderina harziaiuun BCC5828, a mycoparasitic fungus and an enzyme of the chitinase enzyme complex, N- acetylglucosaminidase were tested as a biocontrol agent to control the development of three three pathogens, Fusarium oxysporum, Rizoctonia solani,Pythium aphaniderinatum. Initially Trichoderina strain was grown in liquid medium in shake flasks culture was induced to produce of N- acetylglucosaminidase enzyme in the presence of chitin. In a bioreactor the enzyme production was doubled with the same level of Chitin 1% as compared to shake flask cultures. The enzyme was purified by ammonium sulphate precipitation followed by CM cellulose chromatography, DEAE chromatography and Sephadex G-50 gel filtration. Overall a 32 fold increased in enzyme purity was obtained. In order to characterize the molecular weight of the N-acetylglucosaminidase enzyme was determined by SDS PAGE analysis and the was found to have a molecular weight of 35 KDa Maximal activities were determined at pH 6 and optimum temperature range of 50 °C to 60°C. The enzyme is stable at 25°C and 37°C and enhanced activity was obtained with iron salts. The K,,, and V max value were 4.15mM and 2.O4 mol/min of substrates. The enzyme was not found to be very effected as a biocontrol agent in well diffusion test done on three plant pathogens. However the fungi itself was found be very active and can be used as a good biocontrol against for the three plant pathogens studied
650		0	_aTrichoderma
650		0	_aSoilborne plant pathogens _xBiological control
700	1		_aRakshit, Sudip Kumar, _eChairperson
700	1		_aJindal, Vinod Kumar, _eExamination Committee
700	0		_aAthapol Noomhorm, _eExamination Committee
710	2		_aJoint Japan I World Bank Graduate Scholarship Program, _eScholarship Donor
710	2		_aAsian Institute of Technology Fellowship, _eScholarship Donor
810	2		_aAsian Institute of Technology. _tThesis ; _vno. FB-05-21
856			_3Full-Text _uhttp://203.159.5.9/ait-thesis/detail.php?q=B12474
907			_a.b11991598 _bmnait _cu
902			_a250326
998			_b0 _c060712 _dm _ea _fu _g0
945			_lmnait
945			_lmnarc
942			_c22
942			_c40
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