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035			_a.b12007614
099		9	_aAIT Diss. no.EV-07-8
100	0		_aVitchayut Tupwongse
245	1	0	_aAssessment of metals contents in a water reservoir of Northern Thailand
260			_aPathum Thani, Thailand : _bAsian Institute of Technology, _c2007
300			_a166 p. : _bill. (some col.)
490	1		_aDissertation ; _vno. EV-07-8
500			_aA dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management
502			_aThesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2007
520			_aThe assessment of metals concentrations in freshwater resource had been done at Phayao Lake, Phayao province, Northern Thailand, which is a major water resource for drinking water supply, agriculture, and household use. This study was divided into 3 parts which are field survey, the assessment of dietary exposure from fishery products from the lake, and the cytotoxicity testing of the selected metals in two human cell lines. The field survey samplings were carried out in 3 periods (February, May, and August) in 2005. Metal analysis was performed by using inductively coupled plasma mass spectrometer (ICP-MS). Concentrations of Mn and As in lakewater ranged from 40-382 and 0.68-8.79 ug/L whereas the U.S.EPA. secondary 'maximum concentrations limit for drinking water (Mn) and WHO (As) guidelines for drinking water are 50 and 10 ug/L, respectively. Concentrations of some metals (A1, Cr, Mn, and Fe) in water were found to be higher in the area where water flowed from a small river into the lake than in other areas. The highest metal concentrations were found in the period of the dry season (May 2005). Among different sampling sites, the patterns of metal accumulations were different. Estimated consumption of fishery product from the lake was calculated and the results indicated that the concentrations of metals in these products were lower than the recommended average daily dietary intake. Therefore, the consumption of fish and pond snail from this water resource may not pose a risk of metal toxicity. However, monitoring of the levels of Mn and As in lakewater should be carried out routinely so that appropriate prevention of contamination from these toxic metals can be implemented. For In vitro study, the investigation of cytotoxic effects of a mixture of selected metals As, Mn, and Pb in human neuroblastoma SH-SY5Y cells was done. The cytotoxic effect of individual metal in SH-SY5Y cells was studied using XTT cell viability assay. Treatment of As, Mn, or Pb caused a dose-dependent increase in cytotoxicity in SH-SY5Y cells. The lowest concentration that cause less than 5 % cell death was selected, to be used in multiple exposure studies, 0.05 uM (3.75 ug/L) for As, 0.1 uM (20.27 ug/L) for Mn, and 0.5 uM (27.5 u/L) for Pb. It was noted that these selected concentrations of As and Mn were within the ranges found in Phayao Lake. The results demonstrated that single exposure to each metal at the selected concentration had no significant cytotoxic effect whereas the combination of Mn and Pb or As caused a significant increase in cell death (about 15%) in comparison to exposure to a single metal. The combination of all three metals, As, Mn, and Pb significantly increased cytotoxicity to 25% of control in SH-SY5Y cells. These results indicated that simultaneous exposure to non-cytotoxic levels of multiple heavy metals can potentially enhance the risk of toxic effect in in vitro study
650		0	_aReservoirs _zThiland _zPhayao
650		0	_aWater-supply _zThailand _zPhayao
650		0	_aHeavy metals
700	0		_aJutamaad Satayavivad, _eChairperson
700	0		_aPreeda Pakpian, _eExamination Committee
700	0		_aPiyajit Watcharasit, _eExamination committee
710	2		_aChulabhorn Research Institute, _eScholarship donor
710	2		_aMahidol University, _eScholarship donor
710	2		_aAsian Institute of Technology (CRI-MU-AIT), Thailand, _eScholarship donor
810	2		_aAsian Institute of Technology. _tDissertation ; _vno. EV-07-8
856			_3Full-Text _uhttp://203.159.5.9/ait-thesis/detail.php?q=B00532
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