AI SearchBio-ethanol production by simultaneous cassava starch hydrolysis and fermentation using screened microorganisms
Call Number: AIT Thesis no.FB-07-01 Material type:
SeriesSeries: Asian Institute of Technology. Thesis ; no. FB-07-01Publication details: Pathum Thani, Thailand : Asian Institute of Technology, 2007Description: 58 leaves : illSubject(s): Online resources: Dissertation note: Thesis (M.Sc.) - Asian Institute of Technology, 2007 Summary: In the present study, the main aim was to select and characterize microorganisms capable of simultaneous cassava starch digestion and ethanol production. Eight samples were collected from waste water treatment areas of the Siam Modified Starch Co. Ltd., Pathumthani in Thailand. There were thirty seven strains which were able to hydrolyze cassava starch. Eight of these strains were able to produce ethanol simultaneously. The strain of SMS111 gave highest ethanol among screened strains and gave higher level of ethanol production compared to these of standard strain (Saccharomyces diastaticus TISTR 5221 and Saccharomyces cerevisiae TISTR 5049) under the test conditions. Ethanol concentration of 46.5 and 44.66g/l was obtained in modified MRS added with 2% precooked cassava starch at 37°C and 42°C respectively obtained. Ethanol concentration of 44.5 and 43.45 g/l was obtained in modified MRS added with non-cooked cassava starch at 37 °C and 42 °C respectively. The ethanol production was found to be growth associated. Ethanol accumulation was observed during the whole period, with an increased accumulation among the logarithmic growth stage in all tested conditions. The strain SMS1/1 was also able to grow and produce ethanol in non-cooked starch. This is of great significance as there is considerable energy saving possible
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A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
Thesis (M.Sc.) - Asian Institute of Technology, 2007
In the present study, the main aim was to select and characterize microorganisms capable of simultaneous cassava starch digestion and ethanol production. Eight samples were collected from waste water treatment areas of the Siam Modified Starch Co. Ltd., Pathumthani in Thailand. There were thirty seven strains which were able to hydrolyze cassava starch. Eight of these strains were able to produce ethanol simultaneously. The strain of SMS111 gave highest ethanol among screened strains and gave higher level of ethanol production compared to these of standard strain (Saccharomyces diastaticus TISTR 5221 and Saccharomyces cerevisiae TISTR 5049) under the test conditions. Ethanol concentration of 46.5 and 44.66g/l was obtained in modified MRS added with 2% precooked cassava starch at 37°C and 42°C respectively obtained. Ethanol concentration of 44.5 and 43.45 g/l was obtained in modified MRS added with non-cooked cassava starch at 37 °C and 42 °C respectively. The ethanol production was found to be growth associated. Ethanol accumulation was observed during the whole period, with an increased accumulation among the logarithmic growth stage in all tested conditions. The strain SMS1/1 was also able to grow and produce ethanol in non-cooked starch. This is of great significance as there is considerable energy saving possible
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